干扰素-α和γ对人骨肉瘤细胞nm23-H1基因产物表达的诱导作用

REGULATION OF EXPRESSION OF nm23H1 PROTEIN BY INTERFERONS IN HUMAN OSTEOSARCOMA CELL LINE OS732

目的探讨干扰素对骨肉瘤转移的调节作用，为临床应用干扰素防治骨肉瘤转移提供一定实验基础。方法采用人白细胞干扰素（ＩＦＮα）和免疫干扰素（ＩＦＮγ）对体外培养的人骨肉瘤细胞系ＯＳ７３２ｎｍ２３Ｈ１蛋白的表达进行诱导调节，利用ＣｅｌＥＬＩＳＡ半定量法检测其表达水平的变化。结果ＩＦＮα能显著上调ＯＳ７３２细胞ｎｍ２３Ｈ１蛋白的表达水平（Ｐ＜００１）。结论ＩＦＮα可能在一定程度上抑制骨肉瘤细胞的转移潜能。

PURPOSE To explore the regulatory effects of IFN on invasive and metastatic process of osteosarcoma, and provide experimental evidence for clinical diagnosis and treatment of tumor metastasis.METHODS In this study, the changes of nm23H1 protein were halfquantitatively measured by CellELISA in human osteosarcoma cell line OS732 treated by IFNα and IFNγRESULTS The results demonstrated that IFNα has been shown to be capable of significantly increasing the expression of nm23H1 protein (P<0.01) in OS732 in a dosedependant manner, but the effects of IFNγ is not significant (P>005)CONCLUSIONS The findings suggest that IFNα may be able to suppress the metastatic potential of osteosarcoma and play a role in the treatment of patients with osteosarcoma.