埃托啡诱导神经细胞凋亡的实验研究

ETORPHINE INDUCE NEURONAL CELL APOPTOSIS IN VITRO

应用ＭＴＴ比色法观察埃托啡、吗啡等对神经母细胞瘤细胞株ＳＫ－Ｎ－ＳＨ细胞增殖代谢的影响，同时采用ＴＵＮＥＬ原位细胞凋亡检测技术，观察上述药物有无诱发ＳＫ－Ｎ－ＳＨ细胞凋亡的作用。结果表明，埃托啡不但能抑制神经细胞的增殖代谢，并能引发神经细胞凋亡，且该作用不能被阿片受体拮抗剂纳络酮所阻断。而在同样实验条件下吗啡不引起ＳＫ－Ｎ－ＳＨ细胞的凋亡。提示埃托啡诱导神经细胞凋亡的途径可能不通过阿片受体。

The MTT metabolism of neuronl cell line SK-N-SH were investigated ,and cell apoptosis were detected in SK-N-SH cell after treated with etorphine , morphine and naloxone.The results were show that etorphine not only inhibits the MTT metabolism but also induces apoptosis of SK-N-SH cell,which naloxone cannot block . Morphine only reduces the MTT metabolism and cannot cause apoptosis.So those suggest etorphine may induce apoptosis of neuronal cell,which may not be mediated by opiate receptor.