帕金森症模型鼠靶区移植物中的一氧化氮合酶阳性神经元

THE NITRIC OXIDE SYNTHASE-POSITIVE NEURONS IN TARGET GRAFTS IN THE RAT PARKINSON MODEL

用ＮＡＤＰＨ－黄递酶（ＮＡＤＰＨ－ｄ）组织化学方法结合酪氨酸羟化酶（ＴＨ）免疫组化法，对帕金森症模型鼠靶区移植胚腹侧中脑组织后移植区内一氧化氮合酶（ＮＯＳ）阳性神经元进行观察。结果发现：用６－ＯＨＤＡ损坏一侧黑质纹状体系统后，受损侧黑质纹状体内的ＴＨ阳性神经元及神经纤维明显减少，而ＮＯＳ神经元没有变化。将胚腹侧中脑组织移植到受损侧纹状体后，移植区内ＴＨ及ＮＯＳ阳性神经元存活良好，未见ＮＡＤＰＨ－ｄ＋ＴＨ双标记神经元。移植区内ＮＯＳ神经元有３种形态：一种为大型多极细胞，染色深，可能来自胚黑质致密带外侧；另一种为较小的双极中等程度着色的神经元，可能来自胚脚间核；还有一种ＮＯＳ神经元，无突起或仅有一条短的突起，细胞酶活性低，仅局限于胞质内，可能是诱导型的ＮＯＳ神经元。这些结果表明：移植区ＮＯＳ与ＴＨ不共存，黑质纹状体系统的ＮＯＳ神经元对６－ＯＨＤＡ的毒性不敏感，移植区内不仅有来自胚腹侧中脑ＮＯＳ神经元，可能还有诱导型的ＮＯＳ神经元。

Using NADPH-diaphorase (NADPH-d) histochemistry combined with tyrosine hydroxylase(TH) immunocytochemistry, we investigated the nitric oxide synthase (NOS)-positive neurons in target grafting areas in parkinson's disease(PD)model rats. The results showed that after lesioned by 6-OHDA,TH-positive neurons and neurites on lesioned side of nigral-striatal system reduced significantly, while NOS-positive neurons were unchanged. 6 months after implanting fetal dopaminergic neurons into ipsilateral striatum, TH and NOS-positive neurons survived well in grafting area, but no NADPH-d +TH double labelled neurons were found .Three types of different morphologic forms of NOS-positive neurons were found in grafts. One is large ,multipolar ,intensely stained NOS-positive neuron,it may come from fetal lateral part of ventral mesencephalon; The second type is small, moderate stained bipolar neuron, it may come from fetal interpeduncular nucleus; The third type were similar to the inducible NOS neurons with light NADPH-d positive perikarya and one short process or no process . These results indicate that NOS dose not coexist with TH in grafting area. NOS-positive neurons in nigral-striatal system are not susceptible to 6-OHDA toxicity. In target grafts , NOS-positive neurons may not only come from fetal ventral mesencephalon , but also have inducible NOS neurons.