受体相关蛋白羧基端单克隆抗体诱导被动型Heymann肾炎

PASSIVE HEYMANN NEPHRITIS INDUCED BY ANTI-COOH-TERMINAL OF RECEPTOR-ASSOCIATED PROTEIN(RAP) MONOCLONAL ANTIBODY

应用抗受体相关蛋白羧基端１０８个氨基酸多肽单克隆抗体Ｆ２（ＭｏｎｏｃｌｏｎａｌａｎｔｉｂｏｄｙＦ２，ＭｃＡｂＦ２）进行被动型Ｈｅｙｍａｎｎ肾炎（ＰａｓｓｉｖｅＨｅｙｍａｎｎｎｅｐｈｒｉｔｉｓ，ＰＨＮ）诱导试验。Ｗｅｓｔｅｒｎ－Ｂｌｏｔ分析显示，ＭｃＡｂＦ２识别天然膜抗原Ｆｘ１Ａ中分子量为４４ＫＤａ的受体相关蛋白（Ｒｅｃｅｐｔｏｒ－ａｓｏｃｉａｔｅｄｐｒｏｔｅｉｎ，ＲＡＰ），ＳＤ大鼠体内注射ＭｃＡｂＦ２４天后肾皮质免疫组化和免疫电镜发现ＭｃＡｂＦ２在体内能识别肾小球上皮细胞表面抗原，并与之结合形成典型的ＰＨＮ免疫复合物。表明在ＲＡＰ羧基端１０８个氨基酸多肽上存在着Ｈｅｙｍａｎｎ肾炎病理性表型。

The experiment of inducing passive Heymann nephritis(PHN)with anti-COOH-terminal of RAP monoclonal antibody F2(McAb F2)could recognize the 44KDa RAP in the antigens of rat microvillar membranes(fx1A).4days after McAb F2 was injected into SD rats by vein,immunohistochemical and immunoelectron microscopy showed that McAb F2 could recognize and bind to the antigen on the surface of the glomerular epithelial cell,and form typical immune complexes of PHN in vivo.The result indicates that there is a nephritogenic epitope in the COOH-terminal 108 amino acids polypeptide of RAP.