CoX-2在恶性嗜铬细胞瘤中的表达及与VEGF、MVD的关系

Expression of Cyclooxygenase-2 in malignant pheochromocytomas and the relationship between Cyclooxygenase-2 and vascular endothelial growth factor,microvessel density

目的:探讨CoX-2能否成为一种预判恶性嗜铬细胞瘤的指标,并阐明其与VEGF、MVD间的关系。方法:选取经手术治疗且具有完整的临床、病理和随访资料的嗜铬细胞瘤患者存档石蜡标本38例,其中良性组21例,恶性组17例。另取20例正常肾上腺组织作为对照组。采用免疫组织化学技术,检测良、恶性嗜铬细胞瘤及正常肾上腺髓质组织中CoX-2、VEGF的表达情况及MVD表达情况。结果:①CoX-2在恶性组中表达最高,良性组中表达较低,对照组中无表达,恶性组与良性组及恶性组与对照组之间CoX-2的表达差异有统计学意义(P<0.05)。②VEGF在恶性组中呈高表达,VEGF在恶性组中的表达与在良性组和对照组中的表达差异有统计学意义(P<0.05),在良性组和对照组中的表达差异没有统计学意义(P>0.05)。③MVD在恶性组中表达最高,良性组次之,对照组最少,两两之间的表达差异有统计学意义(P<0.05)。④在嗜铬细胞瘤中CoX-2与VEGF的表达呈正相关,CoX-2的表达与MVD呈正相关,VEGF的表达与MVD呈正相关。结论:CoX-2和VEGF有望成为预判恶性嗜铬细胞瘤的一种指标。

Objective：To analyse the value of Cox 2 as marker for malignant pheochromocytomas. Meanwhile, to investigate the relationship between Cox-2 and VEGF, MVD. Methods： Tissue specimens were obtained at operations performed. There were 38 pheochromocytomas （including 21 benign and 17 malignant） and the clinical and pathological data were available. Normal adrenal glands were from 20 patients undergoing nephreetomy for a benign renal tumor. Immunohistochemical technology was performed to detect the Cox-2, VEGF and MVD（marked with CD34）in all patients. Results： Strong Cox 2 expre.ssion was found in malignant pheochromocytomas, whereas weak Cox-2 expression in benign pheochromocytomas and no expression in normal adrenal medulla immunohisto-chemically. There was statistical difference between malignant group and benign group, also between malignant group and normal adrenal medulla group （ P〈0.05）. Strong VEGF expression was found in malignant pheochro- mocytomas, whereas weak expression in benign pheochromoeytomas and in normal adrenal medulla respectively. There was statistical difference between malignant group and benign group, also between malignant group and normal adrenal medulla group （ P〈0.05）. However there was no statistical difference between benign group and normal adrenal medulla group （ P 〉0.05）. High MVD expression was found in malignant pheochromocytomas, whereas low expression in benign pheoehromocytomas and in normal adrenal medulla. There was statistical differ ence between groups （ P〈0.05）. There was positive relationship between Cox 2 and VEGF in pheochromocytomas, also between Cox-2 and MVD. Meanwhile we also found the positive relationship between VEGF and MVD, Conclusions：Cox 2 and VEGF mav become the predictor marker for malignant pheoehromocvtoma.