抗ICAM-1单抗对体外循环下犬心肌的保护作用

Effects of MAB-to-ICAM-1 on canine myocardial injury in cardiopulmonary bypass

目的探讨抗ICAM-1单抗对体外循环下心肌的保护作用及可能机制。方法应用犬体外循环心肌再灌注损伤模型，比较含抗ICAM-1单抗的灌注液干预前后心功能以及心肌组织或血浆中SOD、MDA、CPK、ATP、LA等的变化。结果体外循环再灌注后，心功能减退，心肌组织中MDA、LA增加，SOD及ATP减少，而血浆CPK增加，发生再灌注损伤。抗ICAM-1单抗干预后心功能显著改善，心肌或血浆中原本升高的MDA、CPK、LA下降，而下降的SOD及ATP增加。结论抗ICAM-1单抗可减少体外循环再灌注时的白细胞黏附，降低心肌自由基水平，减轻心肌损伤，产生心肌保护作用。

Objective To elucidate the protective effect of Mab-to-ICAM-1 on myocardial reperfusion injury during cardiopul- monary bypass（CPB）.Methods Dog heart models with ischemia/reperfusion after CPB wem used. The comnary blood was collected and the specimens of myocardium were biopsied before crossclamping and after reperfusion respectively. At the same time points, cardiac function was measured. The contents of malondialdehyde （MDA）, supemxide dismutase （SOD）, lactic acid （LA）, ATP in myocardium and CPK in the blood of coronary sinus were measured. Results Compared with controls, MDA, and LA contents increased, while SOD and ATP levels decreased obviously after reperfusion. Accompanying these changes, cardiac function attenuated. But the contents of MDA, LA decreased and SOD and ATP increased after reperfusion with Mab-to-ICAM-1. At the same time, the cardiac function was improved. Conclusion In the reperfusion injury hearts in CPB, Mab-to-ICAM-1 could reduce the production of oxygen free radical, maintain the microvascular integrity, improve myocardial metabolism and enhance the recovery from cardiac dysfunction.