上皮性卵巢癌 p^(53) 蛋白过度表达的意义探讨

STUDY OF OVEREXPRESSION OF p53 IN OVARIAN EPITHELIAL CARCINOMA

应用抗ｐ５３蛋白的单克隆抗体对３１例上皮性卵巢癌新鲜组织冰冻切片的ｐ５３蛋白表达行ＡＢＣ法免疫组化测定。结果显示，ｐ５３蛋白阳性部位主要位于癌细胞核内，３例标本（９．７％）伴有细胞浆内轻度阳性染色，３１例标本阳性２３例，阳性率为７４．２％。ｐ５３蛋白的过度表达与组织学分级、临床分期有关（Ｐ＜０．０５），与组织学类型无关（Ｐ＞０．０５）。说明ｐ５３基因的突变可发生在上皮性卵巢癌的早期、中期和晚期，并呈逐渐增加趋势，与肿瘤的生长、扩散关系密切。测定ｐ５３蛋白有助于确定肿瘤的分化程度和临床分期。

Immunohistochemical detection of p53 expression using monoclonal antibody to p53 with ABC method were performed on frozen sections of 31 ovarian epithelial carcinoma specimens.The positive staining of p53 was mainly localized in the nuclei and 3 specimens(9.7%) also had slight cytoplasmic positive staining.The p53 positive staining was found in 23 of 31 cases(74.2%).The overexpression of p53 was significantly correlated with tumor's histological grade and clinical stage(P<0.05),and not with the histological types(P>0.05).The mutation of p53 gene may occur at the early,middle and advanced stages of the ovarian epithelial carcinoma and has a increasing tendency.Therefore,it is highly associated with tumor's growth and spread.Detection of p53 is helpful to determine the tumor's differentiation and clinical stage.