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高脂膳食对SR-AⅠ／Ⅱ基因敲除小鼠白色脂肪组织中UCP2 mRNA表达的影响

Effect of high fat diet on UCP2 mRNA expression in white adipose tissue of SR-A Ⅰ/Ⅱ gene knock-out mice
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摘要 目的:观察SR-AⅠ/Ⅱ基因敲除(SR-AⅠ/Ⅱ-/-)小鼠白色脂肪组织中UCP2 mRNA的表达,探讨其与SR-AⅠ/Ⅱ-/-小鼠肥胖易感的关系。方法:应用荧光定量RT-PCR检测小鼠附睾周白色脂肪组织中UCP2 mRNA的表达;图像分析法评估附睾周脂肪细胞形态学变化;酶法检测甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)血脂水平。结果:经12周高脂饲料喂养,SR-AⅠ/Ⅱ-/-小鼠的体重、血脂(TG、TC、LDL-C)水平、附睾周脂肪垫重量、脂肪细胞面积和直径均大于野生(SR-AⅠ/Ⅱ+/+)对照小鼠(P<0.01);白色脂肪组织UCP2 mRNA表达量低于SR-AⅠ/Ⅱ+/+小鼠(P<0.01)。结论:SR-AⅠ/Ⅱ-/-小鼠对膳食诱导肥胖(diet-induced obesity,DIO)易感可能与其白色脂肪组织UCP2 mRNA表达异常有关。 AIM： To observe the relationship between UCP2 mRNA expression in white adipose tissue and diet - induced obesity in SR - A Ⅰ/Ⅱ gene knock - out （ SR - AⅠ/Ⅱ -/- ） mice. METHODS ： Fluorescent quantitative RT - PCR was used to detect UCP2 mRNA expression in mice epididymal white adipose tissue. The cellular morphological changes were analyzed by using image analysis. Serum TG, TC and LDL - C concentrations were measured by enzymatic determination. RESULTS： After fed with high fat diet for 12 weeks, average body weight of SR - A Ⅰ/Ⅱ-/- mice was much higher than that of wild type （ SR - A Ⅰ/Ⅱ -/- ） control mice （ P 〈 0. 01 ）, as well as the serum lipid （ TG, TC, LDL - C） levels, epididymal fat pad weight, adipocyte area and diameter. UCP2 mRNA expression in white adipose tissue in SR - A Ⅰ/Ⅱ-/- mice was significant lower than that in SR - A Ⅰ/Ⅱ-/- control, mice （P 〈 0. 01 ）. CONCLUSION： Abnormal UCP2 mRNA expression may be associated with SR - A Ⅰ/Ⅱ -/- mice susceptive to diet - induced obesity.
作者 刘明斌 凌文华 余小平
机构地区 中山大学公共卫生学院营养系 成都医学院基础医学院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2008年第9期1704-1708,共5页 Chinese Journal of Pathophysiology
基金 广东省自然科学基金资助项目(No.30025037)
关键词 受体 清道夫 肥胖症 解偶联蛋白质类 Receptors, scavenger Obesity Uncoupling proteins
分类号 R363 [医药卫生—病理学]
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参考文献13

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中国病理生理杂志
2008年 第9期

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