丙戊酸钠诱导K562细胞周期阻滞和凋亡并上调p^21WAF1基因mRNA的表达

Sodium valproate induces cycle arrest,apoptosis and elevates p^(21WAF1) mRNA expression in chronic myeloid leukemia cell line K562

目的:探讨丙戊酸钠(VPA)对慢性粒细胞白血病急变期细胞株(K562细胞)细胞周期和凋亡的影响及其可能机制。方法:利用流式细胞仪检测药物处理后的细胞周期和细胞凋亡情况;利用RT-PCR方法检测p21WAF1基因的mRNA的表达。结果:VPA引起K562细胞凋亡增加[(11.47±0.25)%vs(4.77±0.40)%,(P<0.05)]和细胞周期阻滞在G0/G1期[(82.30±9.41)%vs(40.13±2.12)%,(P<0.05)]。同时处理后的细胞中p21WAF1基因mRNA的表达增加[(1.65±0.91)vs(0.25±0.04),P<0.05]。结论:VPA可能通过上调p21WAF1基因,导致K562细胞周期阻滞并引起细胞凋亡。

AIM： To investigate the effects of sodium valproate （VPA） on the cell cycle and apoptosis of chronic myeloid leukemia cell line K562, and to explore the possible mechanisms. METHODS： K562 cells were treated with VPA. Cell cycle and apoptosis were analyzed by flow cytometry. The expression of P^21WAF1 mRNA was detected by RT - PCR. RESULTS ： After treatment with VPA, cell cycle was arrested obviously at G0/G1 phase [ （ 82. 30 ± 9.41 ） % vs （40. 13 ± 2. 12）% , P 〈 0. 05 ]. The apoptotic rate was significantly higher in the cells treated with VPA than that in untreated cells [ （ 11.47% ± 0. 25% ） vs （4. 77% ± 0. 40% ）, P 〈 0. 05 ]. The level of P^21WAF1 mRNA was increased [ （ 1.65 ±0. 91） vs （0. 25 ± 0.04）, P 〈 0. 05 ]. CONCLUSION： VPA induces elevated expression of P^21WAF1 mRNA in K562 cells, resulting in G0/G1 phase arrest and apoptosis in vitro.