GPIF对免疫损伤小鼠T淋巴细胞膜表面共刺激分子表达的影响

Influence of GPIF on the expression of costimulatory molecules lineaged T cells in mice with immunodeficiency in vivo

目的:分析羊胎盘免疫调节因子(GPIF)对BALB/c小鼠T淋巴细胞共刺激表面抗原分子表达及其细胞因子分泌的影响,探讨羊胎盘免疫调节因子免疫促进作用机理。方法:60Coγ-ray辐射所致免疫抑制小鼠连续7 d腹腔注射GPIF,流式细胞分析术分析BALB/c小鼠脾细胞表达CD28+、CD152+单阳性细胞百分率,表达CD4+CD28+、CD8+CD28+、CD4+CD152+、CD8+CD152+双阳性细胞百分率;ELISA法检测小鼠血清IL-2、IFN-γ分泌水平。结果:羊胎盘免疫调节因子显著提高免疫损伤小鼠脾淋巴细胞CD28+、CD4+CD28+、CD8+CD28+阳性细胞百分率(P<0.05,P<0.01),降低CD152+、CD4+CD152+阳性细胞百分率(P<0.05,P<0.01),提高小鼠血清IL-2、IFN-γ分泌水平(P<0.01)。结论:羊胎盘免疫调节因子的免疫促进作用与其调节T淋巴细胞CD28、CD152共刺激分子通路的活化信号传递,降低T淋巴细胞的功能抑制,促进T淋巴细胞的活化有关。活化的T淋巴细胞分泌细胞因子IL-2、IFN-γ,参与细胞因子介导的免疫网络调节。

AIM： To investigate the effects of goat placenta immunoregulating factor （GPIF） on the expression of costimulatory molecules lineaged T cells in BALB/c mice. METHODS： Animal model for immunodeficiency made from BALB/c mice with whole - body irradiation by 5 Gy ^60Coγ-ray was applied for research. The immunosuppressive mice were injected with GPIF for seven days continuously： FACS was applied to analyze the rate of CD28^＋, CD152^＋ , CD4^＋ CD28 ^＋ , CD8 ^＋ CD28 ^＋ , CD4 ^＋ CD152 ^＋ and CD8 ^＋ CD152 ^＋ cells in splenic lymphocytes and ELISA method was employed to measure the amount of IL - 2 and IFN - γ in serum of mice. RESULTS ： GPIF increased the percentage of CD28 ^＋ , CD4 ^＋ CD28 ^＋ and CD8 ^＋ CD28 ^＋ cells （ P 〈 0. 05, P 〈 0. 01 ）, and decreased the percentage of CD152 ^＋ （ P 〈 0. 05, P 〈 0. 01 ）, CD4 ^＋ CD152 ^＋ cells （P 〈0. 05, P 〈0. 01 ） in splenic lymphocytes of immunosuppressive mice significantly. GPIF increased the content of IL- 2 and IFN- γ in serum of mice simultaneously （P 〈 0.01 ）. CONCLUSION： Immuno- enhancing effect of GPIF facilitates the costimulation of CD28 pathway, which can activate T cells and accelerate the course of renewing T cell activity. The function of GPIF may have close relationship with an immune network formed by the secretion of IL -2 and IFN -γ and the expression of CD28 and CD152.