养血调肝方对绝经后骨质疏松症影响的实验研究

Experimental Study of the Effect of Yangxue Tiaogan Formula on Postmenopausal Osteoporosis

目的:探讨养血调肝方对去卵巢大鼠模型骨密度及骨代谢方面的影响。方法:将雌性6月龄健康wistar大鼠70只随机分为假手术组、模型组、尼尔雌醇组、六味地黄丸组、养血调肝方大、中、小剂量组。用药4个月后检测各组大鼠骨密度、骨矿含量及血清骨钙素、碱性磷酸酶、抗酒石酸酸性磷酸酶、尿脱氧吡啶酚。结果:养血调肝方能明显地增加去卵巢后大鼠骨密度、骨矿含量;升高去卵巢大鼠血清骨钙素、碱性磷酸酶水平,或降低血清抗酒石酸酸性磷酸酶和尿脱氧吡啶酚水平。结论:养血调肝方能通过促进骨形成或抑制骨吸收增加骨密度、骨矿含量,从而对绝经后骨质疏松症有一定的防治作用。

Objective： To evaluate the effect of Yangxue Tiaogan Formula （YTF） on bone mineral density （BMD） and bone metabolism in ovarieetomized rats. Methods： Seventy 6 - month- old female Wistar rats were randomly divided into 2 groups; the model of postmenopausal osteoporosis （PMOP） group established by removing their ovaries and the sham model group. Meanwhile, the rats in PMOP group were randomly divided into 6 groups： the model group, the nilestriol group, the Liuwei Dihuang Pill （LDP） group, the high--dose group, the middle--dose group and the low--dose group of YTF. After 4 months of treatment with different drugs, the contents of BMD, bone mineral content （BMC）, serum osteoealein, AKP, TRAP and deoxypyridinoline （D-- Pyd） were detected. Results： Compared with the model group, the contents of BMD and BMC were remarkably increased,the levels of serum osteocalcin and AKP were raised and the levels of TRAP and D--Pyd were reduced in YTF groups. Conclusion： YTF can prevent and treat PMOP by way of promoting bone formation or inhibiting bone resorption in the ovariectomized rats.