青藏高原7种嵩草遗传多样性AFLP研究

Research of Genetic Diversity in Seven Kobresia by AFLP Tibet Plateau of China

【目的】从分子水平研究嵩草品种资源之间的遗传多样性,为综合评价青藏高原嵩草种质资源提供依据。【方法】用筛选出的4对E+3/M+3引物对11份嵩草基因组DNA进行AFLP扩增。【结果】共得到164条清晰可辨条带,多态性条带154条,多态性位点百分率为93.96%,嵩草平均Nei’s基因多样性指数为0.2430,Shannon’s多样性指数为0.4012,表明嵩草种质间存在丰富的遗传多样性。通过UPGMA聚类分析,将11个嵩草居群划分为5类。【结论】嵩草的11个自然居群存在丰富遗传多样性,嵩草居群的遗传相似系数与海拔之间没有相关性,嵩草居群的生境的异质性影响遗传分化。

[Objective] This work analyzed the genetic diversity of Kobresia accessions, in the molecular level, and further obtained the helpful information for breeding and germplasm evaluation. [Method] Genomic DNA of Kobresia was amplified with four E＋3 and M＋3 primer combinations with AFLP. [Result] AFLP analysis produced 164 score able bands, among them 154 （93.96%） were polymorphic. The mean Nei＇s gene diversity index （H） was 0.2430, the Shannon＇s information index （I） was 0.4012, indicating that genetic diversity of Kobresia is abundant. The number of 11 Kobresia accessions from Tibetan Plateau can be classified into five groups with cluster analysis based on the UPGMA method. [Conclusion] In general, there is an abundant genetic diversity among Kobresia accessions resources, and the genetic coefficient is unrelated to their geographic latitude. Natural habitats influenc genetic differentiation of Kobresia.