电针对震颤麻痹大鼠中脑黑质和肾上腺髓质酪氨酸羟化酶的影响──免疫荧光组化方法

Influence of Electro-acupuncture on Tyrosine Hydroxylase in Mesencephalone and Adrenal Medulla──Fluorescence of Immunohistochemistry

我们以前的实验证明电针“阳陵泉”和“舞蹈震颤区”等穴，可使震颤麻痹大鼠的旋转次数明显少于对照组。本研究旨在通过免疫荧光组化方法显示电针对动物模型中脑黑质及肾上腺髓质酪氨酸羟化酶（TH）的影响。结果表明：在健侧黑质内可见有TH阳性细胞，电针组的TH阳性细胞数明显多于对照组，而且荧光亮度也比对照组强。在6-羟多巴胺毁损侧的黑质区内，仅在电针组见到个别TH阳性细胞及纤维。双侧肾上腺髓质内均有TH阳性细胞反应，电针组的荧光反应强于对照组，结果提示电针可能使TH活性增强。

Our previous research has shown that electro-acupuncture(EA) on "Yan.glinguan (GB 34) and "Wudaozhenchanqu" points could make the tinles of rotation of rat model of parkinson's disease (PD)reduce remarkely than that of control group. This research aimed at studying the influence df EA on tyrosine hydroxylase(TH) in dopaminerngic neurons pf substantia nigra(SN) of rnidbrain of PD rat.The result showed that some of TH-immunoreactive(iR) cells were found in normal side of SN while lesioned side of SN only contained very few of iR cells. Lots of iR cells appeared in adrenal medulla The reaction of immunqfluorescence of TH in both SN and adrenal medulla of EA group was strongel than that of control group. The result suggests that EA may regulate the activity of TH.