摘要
目的探讨胆管上皮细胞缺血再灌注损伤的机制及三七总皂甙在大鼠胆管细胞I/R损伤中的作用。方法采用大鼠原位部分肝缺血再灌注模型。分4组观察在不同条件下胆管细胞及肝细胞的病理形态及超微结构变化及三七总皂甙对此缺血再灌注损伤的影响。结果缺血再灌注组胆管细胞损伤更重,氧自由基生成更多,三七总皂甙预处理保护组胆管损伤明显减轻。结论缺血再灌注损伤对胆管细胞的损伤较肝细胞更重,三七总皂甙预处理可减轻该损伤,其机制可能为抑制氧自由基生成。
Objective To explore the mechanisms of ischemia-reperfusion（I/R） injury of the bile duct and the effect of panax notoginseng saponin（PNS） on ischemia-reperfusion injury of the bile duct. Methods The model of partly rat-hepatic I/R injury was adopted. In four groups,the pathological changes of the bile duct cells and hepatocyte were observed through light microscope and electronmicroscope. And the effective protection of PNS against ischemia-reperfusion injury was investigated. Results The injury of bile duct cells was more serious and more oxygen free radicals were produced. The injury was slight in the group of PNS pretreatment. Conclusion It is more serious for bile duct cells than hepatocyte in I/R injury. PNS pretreatment can provide effective protection against liver ischemia-reperfusion injury. The mechanisms may be depressing the production of oxygen free radical.
出处
《重庆医学》
CAS
CSCD
2008年第16期1787-1788,共2页
Chongqing medicine
