RP-HPLC法测定人血浆中苯巴比妥的浓度

Determination of Serum Concentration of Phenobarbital by RP-HPLC

目的：建立以反效高效液相色谱法测定人血浆中苯巴比妥浓度的方法。方法：色谱柱为C18，流动相为甲醇-水（44：56），检测波长为205nm，流速为1．0mL·min^-1，柱温为35℃。结果：苯巴比妥血药浓度在5．08-63．50μg·mL^-3范围内线性关系良好（r=0．9999）；平均相对回收率为99．75％，平均提取回收率为96．34％；日内、日间RSD均〈4％。结论：本方法操作简便、快速、准确，可用于临床治疗药物监测。

OBJECTIVE： To determine the concentration of phenobarbital （PBB） in serum by RP-HPLC. METHODS： The analytical column was C18. The mobile phase consisted of methanol - water （44 ： 56） at a flow rate of 1,0 mL · min^-1. The detection wavelength was set at 205 nm and the temperature of column was set at 35℃, RESULTS： A good linear relationship was obtained for PBB at a concentration range from 5.08μg · mL^-1 to 63.50μg · mL^-1 （r = 0,999 9） . The mean relative recovery was 99,75% and the mean extraction recovery was 96.34%, The intra - day RSD and the inter - day RSD were all less than 4%. CONCLUSION： This method is simple, rapid, accurate, and suitable for therapeutic drug monitoring.