琼脂糖水凝胶神经支架的研究

Preparation studies of material for Agarose hydrogel

目的：以琼脂糖水凝胶为材料，制备一种神经组织工程支架。方法：琼脂糖水凝胶溶于双蒸水中，利用自制的模具制备支架，冷冻干燥。经显微镜下观察测量其孔隙大小等指标。测试0．01mol·L^-1磷酸盐缓冲液（PBS）中10天的体外降解率，并埋入SD大鼠皮下看其与周围组织反应。结果：制备出的支架具有半透性外壳及高孔隙率的内部海绵状结构，其微孔直径为40～125μm，体外降解率为5．17％，与周围组织基本无毒性反应。结论：琼脂糖水凝胶作为神经组织支架材料，具有一定的应用潜力。

Objective： To produce repairing nerve scaffolds for nerve tissue engineering by Agarose hydrogel. Methods.Firstly, the Agarose was dissolved in double distilled water to get the gelatin. Scaffolds were made from the gelatin by using self-made nerve tissue engineering tooting. Then the scaffolds were freeze-dried. Secondly, by using Microscope, the ultra-microstructure of the scaffolds was observed to the diameters,porosity rate of the scaffolds were measured also. Their degradation rate in 0. 01 mol/L PBS solution for 10 days were observed. Results：The scaffolds had semipermeable chitiosan-crust and spongy collagen core. The average diameter of the scaffolds was 40-125μm and the degradation rate of the scaffolds was 5.17% respectively. Conclusion：The nerve scaffolds have the potential to be applied to nerve tissue engineering.