重组人表皮细胞生长因子对人皮肤细胞增殖作用的研究

Study of the recombinant human epidermal growth factor(rhEGF) on the proliferation of epidermal cells and wound healing

目的：观察重组人表皮生长因子（recombinant human epidermal growth factor，rhEGF）对人表皮细胞增殖的变化。方法：运用包皮环切术后收集培养角质细胞，不同浓度的重组人表皮细胞生长因子处理细胞，MTT法测细胞生长率；流式细胞仪检测细胞周期以及蛋白的表达。结果：5～10ng·ml^-1重组人表皮细胞生长因子可促进人表皮细胞细胞生长。流式细胞仪检测10ng·ml^-1重组人表皮细胞生长因子处理后细胞增殖明显，S和G2／M期细胞数明显增加。结论：重组人表皮细胞生长因子可促进细胞生长以及切口愈合。

Objective. To observe proliferation of epidermal cells of recombinant human epidermal growth factor （rhEGF）. Methods： Epidermal cells from circumcision were cultured, and treated by different concentrations of. By using MTT, , cell growth rate was studied. The cell cycle and the relative protein expression were studied by flow cytometry （FCM）. Results： rEGF （5-100ng· ml^-1） may promote human epidermal cell growth significantly, the effect was most significant by 10ng· ml^-1 rhEGF. More cells were in S and G2/M phase when treated with 10 ng· ml^-1 rhEGF by FCM. Conclusion：Recombinant human epidermal growth factor can promote cell growth and healing of incision.