川崎病和败血症患儿血浆可溶性P-选择素水平检测

Determination of plasma soluble P-selectin levels in children with Kawasaki disease and septicemia

目的:探讨血浆可溶性P-选择素(sPS)水平的变化对小儿川崎病(KD)和败血症的临床诊断价值。方法:应用ELISA双抗体夹心法检测40例KD和40例败血症患儿治疗前后血浆sPS水平的变化,以30例健康儿童为对照组。结果:治疗前后KD组((62.67±4.82)μg/L,(57.96±7.55)μg/L)和败血症组((65.67±6.82)μg/L,(15.46±6.82)μg/L)患儿的血浆sPS水平均明显降低,且均高于对照组((7.14±2.16)μg/L);治疗后KD组高于败血症组(P均<0.01)。结论:sPS参与了KD和败血症发病的急性炎症反应,治疗后血浆sPS水平持续较高可作为诊断KD的参考指标之一。

Aim ： To explore the clinical diagnostic value of plasma soluble P-selectin （sPS） levels in children with Kawasaki disease （KD） and septicemia. Methods：sPS was measured by using double antibody sandwich enzyme linked immunosorbent assay （ELISA） in 40 patients with KD, 40 patients with septicemia and 30 healthy children. ResultS：Before and after treatment, all the plasma sPS levels in children with KD （ （62.67 ± 4.82） μg/L, （57.96 ± 7.55 ） μg/L） and septicemia（ （65.67 ±6.82） μg/L, （ 15.46 ± 6.82） μg/L） had significant difference, and were significantly higher than those in healthy children （ （7.14 ± 2.16） μg/L） ; after treatment, the plasma sPS levels in the KD group were significantly higher than that in the septicemia group （ all P 〈 0.01 ）. Conclusion ： sPS participates in the acute inflammatory reaction of KD and septicemia. After treatment, the plasma sPS level remaining higher in children can be taken as one of the diagnostic markers for KD.