不同剂量HgCl_2与CdCl_2联合对去卵巢大鼠的雌激素样作用

Research on the estrogenic conjoint effect of mercury chloride with cadmium chloride

目的:探讨不同剂量HgCl2与CdCl2联合对去卵巢大鼠的雌激素样作用。方法:66只雌性去卵巢SD大鼠随机分为11组,每组6只,分别给予低、中、高剂量的HgCl2(0.04mg/kg、0.20mg/kg和1.00mg/kg)和CdCl2(0.08mg/kg、0.40mg/kg和2.00mg/kg)及低剂量HgCl2联合低、中、高剂量的CdCl2,阴性对照组给予蒸馏水,阳性对照组给予雌二醇,连续3d,检测各组大鼠的子宫脏器系数和嗜银染色颗粒数。结果:与阴性对照组比较,高剂量HgCl2组、高剂量CdCl2组及低剂量HgCl2与低、高剂量CdCl2联合组大鼠的子宫脏器系数升高,Ag-NORs颗粒数增加(P<0.05);与对应剂量CdCl2组比较,低剂量HgCl2与低剂量CdCl2联合组的子宫脏器系数升高,Ag-NORs颗粒数增加(P<0.05)。结论:低剂量HgCl2与CdCl2联合可能具有雌激素样作用。

Aim-To evaluate the estrogenic conjoint effect of HgCl2 with CdCl2. Methods：A total of 66 SD rats were allocated into 11 groups, and were given low, middle, high dose HgCl2 （0.04,0.20, and 1.00 mg/kg） , low, middle, high dose CdCl2 （0.08,0.40,and 2.00 mg/kg） , and low dose HgCl2 combined with low, middle, high doses CdCl2 ,respectively. The negative and positive control groups were given distilled water and E2. After 3 days, the growth of the uterus and Ag-NoRs were determined. Results：Compared with negative control group,high dose HgCl2 , high dose CdCl2 and low dose HgCl2 combined with low or high dose CdCl2 could stimulate the growth of uterus, and the means of Ag-NORs increased;compared with low dose CdCl2 group,the low dose HgCl2 combined with low dose CdCl2 could stimulate the growth of uterus, and the means of Ag-NORs increased. Conclusion ：Low dose HgCl2 and CdCl2 combination has estrogenic conjoint effects.