拓扑替康对HL-60细胞增殖的抑制作用

Growth inhibitory effect of TPT on HL-60 cells

目的:探讨拓扑替康(topotecan,TPT)对人髓系白血病细胞HL-60的增殖抑制作用。方法:采用四甲基偶氮唑盐光吸收法(MTT法)检测不同浓度的TPT(0.05μmol/L、0.10μmol/L、0.15μmol/L、0.20μmol/L及空白对照组)作用HL-60细胞后4h、8h、12h、16h的增殖情况,计算各组细胞的增殖抑制率,并找出TPT作用HL-60细胞的最适时间和最适浓度。倒置显微镜观察细胞生长状态及形态学改变。结果:TPT作用HL-60细胞12h后细胞集落减少,细胞中颗粒及碎片增多。双因素方差分析显示:不同浓度TPT作用不同时间后,对HL-60细胞生长抑制率差异有统计学意义(F时间=312.24,P<0.05;F组别=1110.35,P<0.05)。同一时间点不同浓度的TPT对HL-60细胞的抑制作用随浓度的升高而增强,同一浓度TPT在不同时间点对HL-60细胞的抑制作用随时间的延长而增强,其作用的最适时间和最适浓度分别为12h和0.15μmol/L。结论:拓扑替康能抑制HL-60细胞的增殖,且其抑制作用具有时间和浓度依赖性。

Aim： To investigate the Inhibitory effect of TPT on H L-60 cells proliferation. Methods：With different concentration of topotecon（0.05 μmol/L,0.10 μmol/L,0.15 μmol/L,0.20 μmol/L and control group） ,the HL-60 cells were incubated for 4 h,8 h, 12 h, 16 h, respectively. The inhibitory rate of cell proliferation is assayed by MTT assay. Then, optimal concentration and time was found. At the same time ,growth appearance and morphology of the cells were observed with microscope. Results： The morphology of HL-60 cell changed after the treatment of TPT, such as HL-60 cellcolony decrea- sing, while the cells granule and fragments increased. On the same time spot, the inhibition effect of TPT on HL-60 cells was strengthened with the increase of concentrations. For the same concentration of TPT on different time spot, the inhibition effect of TPT on HL-60 cells was strengthened with the increase of time expansion. The optimal concentration was 0.15 μmol/L of TPT and the optimal time was 12 h among all groups. Conclusion： TPT is able to inhibit the HL-60 cells growth by time- and dose-dependent manners.