冠心病患者外周血中p选择素和淀粉样蛋白A的变化

Expressions of serum SAA and CD62p in the patients with coronary heart disease

目的:观察冠心病患者外周血中p选择素(CD62p)及淀粉样蛋白A(SAA)的变化,并探讨其临床意义。方法:采用酶联免疫吸附法(ELISA)和流式细胞术(FCM)检测30例稳定性心绞痛(SAP)组、30例不稳定性心绞痛(UAP)组、50例急性心肌梗死(AMI)组和30例健康对照组外周血中SAA水平和CD62p的阳性百分率。结果:SAP组、UAP组、AMI组的SAA水平依次升高(分别为(6.32±1.74)mg/L,(9.52±2.37)mg/L,(420.60±158.11)mg/L),均明显高于健康对照组((2.75±1.58)mg/L),各组之间比较差异有统计学意义(F=131.79,P<0.05);SAP组、UAP组、AMI组的CD62p阳性百分率依次升高((18.31±4.74)%,(36.52±15.81)%,(62.35±16.60)%),均明显高于健康对照组((10.52±3.16)%),各组之间比较差异有统计学意义(F=18.90,P<0.05);SAP组、UAP组、AMI组血浆中CD62p阳性表达率与SAA水平均呈正相关(r分别为0.432、0.512、0.548,P<0.05)。结论:SAA和CD62p在冠心病的发生发展中起着重要的作用,二者联合检测对于冠心病患者病情的评估具有较好的临床应用价值。

Aim： To observe the chages of serum CD62p and serum amyloid A （SAA） in the patients with coronary heart disase and discuss its clinical significance. Methods ：The levels of serum SAA in the 30 cases with stable angina pectoris （SAP group）, 30 cases with unstable angina pectoris （UAP group）, 50 cases with acute myocardial infarction （AMI group） and 30 cases with healthy controls were detected by enzyme linked immunosorbent assay （ELISA） ; The positive expressions of serum CD62p in the 30 cases with SAP, 30 cases with UAP, 50 cases with AMI and 30 cases with health con- trol was detected by flow cytometry （FCM）. Results： The levels of SaA rised in SAP group, UAP group and AM] group by turns and were all higher than heahhy control group, There was a statistical signicance among all groups;The positive expressions of CD62p rised in SAP group, UAP group and AMI group by turns and were all higher than healthy control group, There was a statistical signicance among all groups; There was a positive correlation between the positive expressions of CD62p and the levels of SAA in SAP group, UAP group, AMI group. Conclusion： SAA and CD62p play the important roles in development, occurrence of coronary heart disease. The united detections of the two parameters have fine clinical application values for the evaluations of pathogenetic condition of coronary heart disease＇s patients.