葡萄糖酸锑钠对NK92mi细胞杀伤和蛋白酪氨酸磷酸酶-1活性的影响

Effect of sodium stibogluconate on cytotoxicity of killer cell line NK92mi

目的:探讨葡萄糖酸锑钠(SSG)对NK92mi细胞的杀伤活性和蛋白酪氨酸磷酸酶-1(SHP-1)活性的影响。方法:取对数生长期的NK92mi细胞,随机分为SSG处理组和未处理组,SSG处理组在含有不同质量浓度SSG的培养基中培养16h,用乳酸脱氢酶释放法检测NK92mi细胞对K562细胞的杀伤活性,并用蛋白酪氨酸磷酸酶活性分析法检测经SSG处理后NK92mi细胞中SHP-1的活性。结果:未处理组NK92mi细胞的杀伤活性为(163.12±35.42)LU,经过与1mg/L,10mg/L和100mg/LSSG共同培养后,NK92mi细胞的杀伤活性分别为(210.53±38.35),(353.25±31.99)LU和(771.03±24.80)LU,NK92mi细胞的杀伤活性随SSG质量浓度的增加而显著增高(F=210.16,P<0.05);经1mg/L,10mg/L和100mg/L的SSG处理后,NK92mi细胞中的SHP-1的活性分别降低到未处理组的(57.9±6.3)%,(23.1±5.7)%和(5.2±2.8)%,SHP-1活性随着SSG质量浓度的增加而显著降低(F=81.12,P<0.05)。结论:SSG可能通过降低NK92mi细胞内的SHP-1活性来提高其杀伤活性。

Aim： The effect of sodium stibogluconate （SSG） on NK92mi killing activity and SHP-I activity were investigated. Methods：NK92mi cells were divided into control and experimental groups. The later were treated with SSG at different concentrations for 16 h. The natural killer （NK） activity was assessed by LDH release and the relative activity of SHP-1 was analyzed using PTPase assay. Results： The natural killer activity NK92mi cells in control group was （ 163.12 ± 35.42） LU. After treated with 1 mg/L,10 mg/L and 100 mg/L of SSG, the NK killing activity were （210.53 ± 38.35） LU, （ 353.25 ± 31. 99） LU and （77. 03 ± 24.80） LU ,respectively. NK killing activity was significantly enhanced by SSG （F = 210.16,P 〈 0.05）. The PTPase activity of SHP-1 after treated with SSG at 1 rag/L, 10 mg/L and 100 mg/L was decreased to （57.9 ± 6.3 ） % , （23.1 ± 5. 7 ） % and （5.2 ± 2.8） % of control group, SHP-1 activity was significantly inactivated by SSG（ F = 81. 12, P 〈 0.05 ）. Conclusion： The SHP-1 activity as inactivated and NK killing activity of NK92mi cells was enhanced after SSG treatment.