慢性病毒性乙型肝炎患者血清IL-2、IL-6水平与T细胞亚群检测

Determination of serum levels of IL-2,IL-6 and T lymphocyte subsets in patients with chronic hepatitis B

目的:研究慢性病毒性乙型肝炎患者IL-2、IL-6、T细胞亚群的变化及意义。方法:采用ELISA方法测定71例慢性病毒性乙型肝炎患者(轻度24例,中度28例,重度19例)和51例正常对照血清IL-2、IL-6水平,用间接免疫荧光法测定T细胞亚群,用荧光定量PCR法对HBV-DNA进行扩增,并根据PCR扩增结果分为HBV-DNA阳性组与HBV-DNA阴性组。结果:随病情加重,CD4+细胞百分率与CD4+/CD8+呈下降趋势,而CD8+细胞百分率和血清IL-6水平呈上升趋势(P均<0.05);与HBV-DNA阴性组相比,HBV-DNA阳性组血清IL-2水平、CD4+/CD8+明显降低,血清IL-6水平、CD8+细胞百分率明显升高(P均<0.05)。结论:慢性病毒性乙型肝炎患者存在T细胞亚群和细胞因子的失衡,IL-2、IL-6与T细胞亚群的变化能反映机体免疫损伤情况,对判断患者病情和预后有一定价值。

Aim ： To study the changes of IL-2, IL-6, T lymphocyte subsets in patients with chronic viral hepatitis B. Methods：Seventy-one chronic hepatitis B patients and fifty-one normal controls were collected in the study, and the patients were categorized into three groups according to the degree of hepatic injury： light degree （n = 24）, medium degree （n = 28） and severe degree （n = 19）. Serum IL-2 and IL-6 levels were determined by double antibody sandwich ELISA, T-lymphocyte subsets were determined by indirect immunofluorescent assay,and serum HBV-DNA levels were determined by fluorescent quantitative PCR. The patients were categorized into HBV-DNA positive group and HBV-DNA negative group according to the results of PCR. Results： Along with the aggravation of hepatic injury, the CD4＋ percentage and CD4 ＋/ CD8＋ ratio decreased gradually and the IL-6 level and CD8＋ percentage increased gradually. In HBV-DNA positive group, the IL-2 level and CD4 ＋/CD8 ＋ ratio significantly decreased, IL-6 level and CD8 ＋ percentage significantly increased compared with HBV-DNA negative group. Conclusion： T lymphocyte subsets and cytokines are abnormal in patients with chronic viral hepatitis B. Detection of IL-2, IL-6 and the T lymphocyte subsets can indicate the patients immune function and is valuable to some extent in monitoring the status of disease.