蛋白酶抑制剂对弓形虫发育和感染影响的初步研究

Preliminary research on effects of protease inhibitor on development and inflection of Toxoplasma gondii

目的探索蛋白酶抑制剂对弓形虫发育和入侵宿主细胞能力的影响,寻找弓形虫发育和入侵中起关键作用的蛋白酶及其抑制剂,为弓形虫病防治研究提供科学依据。方法建立弓形虫病BALB/c小鼠模型,观察体外培养和小鼠体内速殖子发育在蛋白酶抑制剂作用下的形态变化,观察蛋白酶抑制剂对弓形虫感染鼠存活时间的影响。结果加入蛋白酶抑制剂,体外培养和小鼠体内发育的速殖子部分发生形态变化;蛋白酶抑制剂+弓形虫感染鼠存活时间明显延长,弓形虫感染+蛋白酶抑制剂治疗后的小鼠生存时间分别为Aprotinin组(204.8±5.2)h,Cystatin组(288.4±4.3)h,Pepstatin组(289.5±6.2)h,对照组(91.2±6.7)h,差异有统计学意义(P<0.05)。结论丝氨酸蛋白酶、半光氨酸蛋白酶、天冬氨酸蛋白酶在弓形虫发育和入侵宿主细胞中起关键作用,相应的蛋白酶抑制剂对弓形虫发育和入侵细胞有明显影响,尤以Pepstatin的作用更显著。

Objective To explore the influence of protease inhibitor on Toxoplasma gondii development and ability of invading the host cell,search the proteinase and its＇ inhibitor which are the key factors in the course of development and invade,and provide the scientific basis for preventing and researching the toxoplasmiasis.Methods Established a little mouse model of BALB/c and observed the culture in vitro and the change of protease inhibitor＇s shape in the body of this little mouse during the period of tachyzoite development and the effect of protease inhibitor to the inflected little mouse＇s existing time.Results There is rather a certain proportion change of the shape between the culture in vitro with adding protease inhibitor and tachyzoite development in the body of a little mouse.The existing time of the inflected little mouse ＋ protease inhibitor is much longer.The exsiting time of the little mouse after the cure by arciform worm＇s inflection ＋ protease inhibitor is aprotinin group（204.8±5.2） h,Cystatin group（288.4±4.3） h and comparison group（91.2±6.7） h,which have the significance of statistias（P〈0.05）.Conclusion Serine protease,cysteine protease and aspartic acid protease are the three key factors in the course of arciform worm＇s development and invading cells.The relevant types of protease inhibitor all have a obvious effect to the arciform worm＇s development and invading cells,especially the obvious function of Pepstatin.