摘要
目的研究曲普瑞林对子宫内膜癌细胞株HEC-1-B的抑制作用及其分子作用机制。方法以不同浓度曲普瑞林(10-9~10-5mol/L)体外作用于子宫内膜癌细胞株HEC-1-B,用四甲基偶氮唑蓝(MTT)法检测细胞抑制率,免疫组织化学检测细胞中P21WAF1/CIP1(简写为P21)蛋白表达,并用病理图像分析软件进行半定量分析。反转录-聚合酶链反应(RT-PCR)法检测P21mRNA的表达。结果①当曲普瑞林浓度为10-9mol/L时,癌细胞即受到抑制,抑制率为36.8%;随着曲普瑞林浓度的增大,抑制率渐高。到浓度为10-5mol/L时抑制率上升至57.4%,与对照组比较差异有统计学意义(P<0.05)。②浓度从10-9mol/L^10-5mol/L的曲普瑞林作用72 h后,P21的表达有不同程度的上升,与对照组相比,差异有统计学意义(P<0.05)。结论①曲普瑞林在体外对HEC-1-B细胞可能有直接的抑制作用,且呈剂量依赖关系。②曲普瑞林抑制HEC-1-B细胞增殖,其分子作用机制可能与上调P21蛋白表达有关。
Objective To study if Alarelin can inhibit cell proliferation of human endometrial cancer cell line HEC-1-B and to explore its molecular mechanisms. Methods HEC-1-B cells were cultured in DMEM medium with alhrelin. The inhibited activity of cells was detected by MTT methods. The expression of p21WAF1/CIP1 proteins was detected by immunohistochemical methods, the semi-quantity of proteins expression were analyzed by pathological image analysis software, The expression of p21WAF1/CIP1 mRNA was detected by reverse transcription-polymerase chain reaction. Results ①The growth of HEC-1-B cells was inhibited when the lowest Alarelin concentration 10^-9mol/L was given, the inhibitory rate was 36.8 %. The inhibitory rate increased with the rising of Alardin concentrations. When Alarelin concentration was 10^-5mol/L, the inhibitory rate increased to 57.4 %, the difference was significant （P 〈 0.05 ）. ②After 72 h with 10^-9mol/L to 10^-5mol/L Alarelin, the level of p21 increased. The difference was significant （P〈0.05）. Conclusion ③Alarelin might have directed inhibitory response on HEC-1-B cells in vitro and the effect was dose-dependent. ④Up-regulation of p21 protein may be one of the mechanisms through which Alardin induced growth inhibition of HEC-1-B cells.
