咳喘宁对支气管哮喘大鼠肺组织血管内皮生长因子含量的影响

Effect of Kechuanning on Vascular Endothelial Growth Factor in Lung Tissue of Bronchial Asthmatic Rats

目的:观察咳喘宁对支气管哮喘大鼠肺组织血管内皮生长因子(VEGF)含量的影响。方法:40只SD大鼠随机分为正常组、模型组、咳喘宁高剂量组(27g生药/kg体重)、咳喘宁低剂量组(13.5g生药/kg体重)、桂龙咳喘宁对照组(0.41g/kg体重),每组8只。除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4周后处死大鼠,采用酶联免疫法检测肺组织VEGF含量,采用HE染色观察肺和气管病理组织学变化。结果:与正常组比较,模型组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织VEGF含量明显增加(P<0.01);与模型组比较,各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数和肺组织VEGF含量(P<0.01),咳喘宁高低剂量组优于桂龙咳喘宁组(P<0.05或者P<0.01)。结论:咳喘宁通过降低肺组织VEGF含量,抑制气道重塑。

Objective： To observe the effect of Kechuanning on the vascular endothelial growth factor（VEGF） in lung tissue of bronchial asthmatic rats. Methods： 40 rats were randomly divided into 5 groups： the normal group, the model group, Kechuanning high - dose group （ 27g/kg）, Kechuanning low - dose group （ 13.5g/kg）, Guilong Kechuanning control group（0. 45g/kg） ,8 rats in each group. The bronchial asthmatic model was established by egg protein sensibilization and long - term inhalation provocation. The rats of each treatment groups were lavage administration each day from the first time of provocation to execution. After 4 weeks of treatment,the rats were killed and lung tissue were taken to dyeing of HE to be observed. The content of VEGF in lung tissue were determined by ELISA. Results ： Compared with the normal group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomococyte, and the content of VEGF in lung tissue were increased（ P 〈 0.01 ） ; Compared with the model group, the thickness of bronchus wall and bronchus smooth muscle, tile numbers of eosinophile granulocyte and leukomonocyte, the content of VEGF in lung tissue were decreased （ P 〈0.01 ）, and Kechuanning high -dose and low -dose group surpass control group（ P 〈 0.05 or P 〈 0.01 ）. Conclusion： Kechuanning could decrease the content of VEGF in lung tissue to restrain airway remolding.