软脉煎对白介素-6单核细胞趋化蛋白-1表达水平的影响

Investigate the Effection of Chinese Medicine Ruanmai Jian on the Level of IL-6 and Mcp-l in Rabbits

目的:探讨具有补肾益气作用的软脉煎对与家兔胸主动脉血管平滑肌细胞增生有关的白介素6(IL-6)、单核细胞趋化蛋白-1(MCP-1)的影响。方法:用贴块法培养家兔胸主动脉血管平滑肌(VSMC),用药物血清学方法将药物血清作用于细胞模型。设立正常组、模型组、软脉煎5倍、10倍、20倍成人剂量组和辛伐他汀10倍成人剂量组。夹心酶联免疫吸附法观察白介素-6、单核细胞趋化蛋白-1的表达。结果:软脉煎能够降低凝血酶诱导的家兔VSMCs白介素-6、单核细胞趋化蛋白-1的表达。结论:软脉煎可能是通过调节血管平滑肌细胞释放白介素-6、单核细胞趋化蛋白-1来抑制血管平滑肌增生。

Objective： To investigate the effect of Chinese medicine Ruanmai Jian on hyperplasia-derived thrombin of VSMCs in rabbits, such as IL- 6 and MCP- 1. Methods： Rabbit aortic vascular smooth muscle cells were cultured by a substrate - attached explant method. Thrombin eliced hyperplasia of AVSMCs andthe expression of cytokine. Methods ： serum was acted on the cell model by serum pharmacologic method. Set up normal group, model group, simavastatin group and group of different Ruanmai J ian dosage. IL - 6 protein and MCP - 1 protein were examined by sandwich EI,ISA ; Result - In the model group, rabbit AVSMCs showed a heavily pathological changes correspond to AS, Ruanmai Jian could inhibiteffectively the expression of IL - 6 （ P 〈 0.01 ）, MCP - 1 （ P 〈 0.01 ）. Conclusion ： Ruanmai Jian could restrain the hyperplasia of AVSMCs by adjusting release of IL -6 and MCP - 1 from VSMCS.