鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶双反义腺病毒对食管癌细胞凋亡和抑制侵袭的作用

Apoptosis effectsand inhibitory growth effect of biantisense virus Ad-ODC-AdoMetDCas on esophageal cancer cell Eca109

目的观察鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶双反义腺病毒对食管癌Eca109细胞凋亡作用及抑制细胞侵袭的影响。方法采用Western印迹检测腺病毒载体对食管癌Eca109细胞中ODC和AdoMetDC蛋白表达；应用噻唑蓝（MTT）比色法测定观察Ad-ODC-AdoMetDCas对食管癌Eca109细胞生长增殖的影响；应用原位末端标记（TUNEL）法和Matrigel侵袭实验分别观察Ad-ODC-AdoMetDCas对食管癌Eca109细胞凋亡作用的影响和侵袭活性的改变。结果Ad-ODC-AdoMetDCas可明显抑制食管癌Eca109细胞中ODC和AdoMetDC基因表达（P〈0．05）。MTT法实验表明，Ad-ODC-AdoMetDCas对食管癌Eca109细胞生长增殖有显著抑制作用（P〈0．05）。TUNEL标记检测Matrigel侵袭实验结果显示，Ad-ODC-AdoMetDCas可明显引起食管癌Eca109细胞凋亡（P〈0．05），可显著降低食管癌细胞Eca109的体外侵袭能力（P〈0．05）。结论Ad-ODC-AdoMetDCas能显著促进细胞凋亡，抑制食管癌细胞侵袭。

Objective To study the inhibitory effects of Ad-ODC-AdoMetDCas on polyamine biosynthesis,esophageal cancer cell growth and apoptosis. Methods Western Blot were used to detect ODC and AdoMetDC expression in Eca109 cells. Adenovirns-mediated gene transduction efficiency was assessed with counting GFP-positive cells using MTT. The malignant phenotype of Eca109 cells was assessed by growth curve. TUNEL was used to analyze cell apoptosis. Eca109 cell invasion in vitro was detected with Matrigel invasion assay. Results The expression of ODC was inhibited in the infected tumor cells. Ad- ODC-AdoMetDCas could inhibit Eca109 cell growth and invasive ability. TUNEL proved that Ad-ODC- AdoMetDCas can lead to cell apoptosis. Gene transferred tumor cells were shown to possess markedly decreased invasiveness. Conclusion Ad-ODC-AdoMetDCas has significant inhibitory effects on esophageal cancer cell invasion,leads to cell apoptosis and bears therapeutic potential for the treatment of esophageal cancer.