同种异体胰岛及胰腺干细胞来源的胰岛样结构序贯移植治疗糖尿病

Transplantation of allogeneic pancreatic islets and pancreatic stem cells-derived islet-like structures to treat diabetes

目的观察同种异体大鼠胰岛及胰腺干细胞来源的胰岛样结构序贯移植在糖尿病治疗中的作用。方法分离胰腺组织获得胰岛及胰腺导管上皮细胞，将具有干细胞潜能的胰腺导管上皮细胞在体外培养27d。将新鲜分离的胰岛（200±50）个及诱导分化2周的胰腺干细胞来源的胰岛样结构（2×10^6）个序贯移植到糖尿病大鼠的肾被膜下观察大鼠的血糖及生存情况。结果将胰岛及胰腺干细胞来源的胰岛样结构序贯移植到同一糖尿病大鼠3周后血糖仍在5mmol／L水平，对照组血糖无明显下降。结论胰腺干细胞可诱导分化为分泌胰岛素的胰岛样结构，胰岛及胰腺干细胞来源的胰岛样结构序贯移植对大鼠糖尿病有治疗作用。

Objective To observe the transplantation of allogeneic pancreatic islets and pancreatic stem cells-derived islet-like structures in the treatment of diabetes. Methods The pancreatic tissue was isolated to obtain the islets and pancreatic duct epithelial cells. Pancreatic duct epithelial cells which have the potential of stem cells were cultivated in vitro for up to 27 days. The freshly isolated pancreatic islets （200 ± 50） and pancreatic stem cells-derived islet-like structures （2 × 10^6） collected 2 weeks after differentiation in vitro were transplanted under the kidney capsule of the same rats. Blood glucose level and the subsistence state were observed. Results Blood glucose level was 5 mmol/L in diabetic rats 3 weeks after the transplantation of freshly isolated allogeneic pancreatic islets and pancreatic stem cell-derived islet-like structures. In control group, the blood glucose level had significant decrease. Condusion Pancreatic stem cells can differentiate into islet-like structures in vitro. Pancreatic islets and pancreatic stem cells-derived islet-like structures have a potential application to cure diabetes.