广金钱草HPLC指纹图谱的研究

Fingerprint of Desmodium styracifolium by HPLC

目的:采用高效液相色谱法对来自11个地区广金钱草药材进行指纹图谱研究,为广金钱草药材质量控制提供依据。方法:采用Phenom sil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-甲醇-水为流动相进行梯度洗脱,流速1.0 mL/m in,柱温25℃,检测波长272 nm。结果:以11个共有峰为评价指标,精密度和重复性试验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,11批样品相似度大于0.9。结论:此方法精密度、稳定性和重复性良好,有助于广金钱草药材的质量控制。

AIM： The fingerprint chromatograms were established for quality evaluation of Desmodium styracifolium collected from II areas by HPLC. METHODS- The analysis was performed on a Phenomsil C18 column （4.6 mm×250 mm, 5 μm） with acetonitrile-carbinol-water as mobile phase in a gradient mode at a flow rate of 1. 0 mL/min, and at a column temperature of 25℃. The detection of wavelength was at 272 nm. RESULTS ： 11 peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values and relative peak areas were less than 3% in the precision and repeated test, the similarity of I I batches of samples were no less than 0.9. CONCLUSION： The method is reliable and can be helpful on the quality control of Desmodium styracifolium.