消瘀化痰饮对非酒精性脂肪肝大鼠肝脏PPARα表达的影响

Effect of Xiaoyu Huatan Decoction on rat tissue PPARα of nonalchoholic fatty liver disease rat

目的:观察消瘀化痰饮对非酒精性脂肪肝(NAFLD)大鼠肝脏过氧化物酶体增殖物激活受体α(PPARα)表达的影响。方法:采用喂饲高脂饲料的方法复制NAFLD大鼠模型,实验分组为正常对照组、模型组、东宝肝泰对照组和消瘀化痰饮高、低剂量组。提取肝脏总RNA,运用半定量RT-PCR方法观察各组大鼠肝脏PPARαmRNA的表达情况,同时测定各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)和肝组织匀浆TC、TG的含量,并做病理切片。结果:模型组大鼠PPARαmRNA的含量明显降低,血脂和肝脏脂质含量明显升高,肝脏呈明显脂肪变性,经药物治疗后,各治疗组大鼠肝脏PPARαmRNA表达明显增强,血脂和肝脏脂质含量显著降低,肝脂变程度明显减轻。结论:消瘀化痰饮能增强NAFLD大鼠肝脏PPARαmRNA的的表达,可能是其治疗NAFLD的分子机制之一。

AIM： To observe the effect of Xiaoyu Huatan Decoction on the nonalchoholic fatty liver tissue peroxisome preliferator - activated receptorct （PPARct） expression. METHODS： The nonalchoholic fatty liver disease rat model was fed by high fat forage and the rats were divided into five groups： normal control group, model control group, high-dose of Xiaoyu Huatan Decoction group, low-dose of Xiaoyu Huatan Decoction group, Dongbao Gantai control group. Total RNA of liver was extracted, and the expression of PPARctmRNA was analyzed by semi- quantitative RT-PCR method. We determined the contents of total cholesterol （TC）, triglyceride （TG）, free ratty acid （FFA） in serum and the TC, TG in liver tissue homogenate of each group, and the degree of hepatocytic steatosis. RESULTS ： Expression of liver tissue PPARα mRNA in the model group decreased significantly, lipid in blood serum and hepatic tissues increased significantly, liver fat cell greatly denaturalized. After the intervention of medicine, expression of liver tissue PPARα mRNA of each treatment group increased significantly, lipid in blood serum and hepatic tissues decreased significantly, liver fat cell denaturation was improved. CONCLUSION： Xi- aoyu Huatan Decoction can increase the expression of liver tissue PPARαmRNA of rats, It is likely to be one of the important mechanism for treating fatty liver.