摘要
在0.1mol/L的磷酸盐缓冲溶液(pH7.4)中,用微量热法测定了298.15K时漆树漆酶与邻氨基苯酚反应的摩尔反应焓ΔrHm=252.21±3.59kJ/mol,米氏常数Km=7.77×10-3mol/L和表观一级反应速率常数k1=7.23×10-3s-1,及此条件下邻氨基苯酚作底物时的漆酶活性值EA=0.190(IU)。
The total molar enthalpy and the Michaelis constant and the observed first ordor velocity constant of the reaction between the Rhus vernicifera laccase and O aminophenol have been determined at 298.15K by microcalorimetry in 0.1mol/L phosphate salt buffer (pH7.4).ΔrHm=-252.21±3.59kJ/mol and Km =7.77×10 -3 mol/L and k 1=7.23×10 -3 s -1 ,correspond.The enzymatic activity of the laccase have been determined to be EA=0.190(IU) under such conditions by using the O aminophenol as the substrate.
出处
《化学研究与应用》
CAS
CSCD
1997年第5期459-463,共5页
Chemical Research and Application
基金
湖北省教委资助
关键词
微量热法
漆树漆酶
邻氨基苯酚
漆酶
催化氧化
Microcalorimetry,Rhus vernicifera laccase,O aminophenol,Enzymetic reaction