营养和环境因素对小麦根腐菌菌丝生长及产孢的影响

The Effect of Nutritional and Environmental Factors on Hyphal Growth and Sporulation of Helminthosporium sativum

本文报道了一些营养和环境因素对小麦根腐菌菌丝生长和孢子产生的影响.研究结果表明,菌丝生长的适宜碳源为单糖或双糖,氮源为铵态氮,矿质元素为KH_2PO_4,适宜酸度和环境条件为pH6.0和30℃,交替光照培养;孢子产生的适宜碳源为多糖,矿质元素为FeSO_4·7H_2O,适宜酸度和环境条件为pH6.0和20～25℃黑暗培养.氮源对孢子产生的影响较为复杂.天然有机物质麦叶浸出汁和土豆浸出汁既利于菌丝生长,也利于孢子产生.菌丝生长和孢子产生的适宜条件一般呈负相关.

The experimental results showed that the optimum conditions for hyphal growth of Helmenthospcrium sativum(Cochliobolus ,saticus) were monosaccharide or bisaccharide (as carbon source) 2 NH4 +-state nitrogen (as nitrogen source) and KH2PO4 (as mineral nutrition) , the optimum acidity and temperature for pathogen growth were pH 6.0 and 30℃ respectively- and alternatively illuminating culture was necessary. The optimum conditions for sporulation were polysaccharide (as carbon source) , FeSO4 · 7H2O (as mineral nutrition) and other environmental factors. pH 6.0,20-25℃ and dark culturing. The effect of nitrogen source on the sporulation was complex. The extracts of wheat leaves and potatoes were advantageous net only to hyphal growth but to sporulation. The optimum condition: for hyphal growth was negatively correlated with the conditions for sporulation.