继代培养中水稻愈伤组织核酸、蛋白质以及酶的动态变化

A Study on Dynamic Changes of Uncleic Acid, Protein and Zyme of Rice Callus in Continuous Culture

水稻愈伤组织在继代培养过程中产生分化能力不同的无性系,在分化培养基上的分析结果表明,具有分化能力的无性系DNA、RNA、蛋白质含量明显高于丧失分化能力的无性系;DNase、RNase活性也有差异,但不明显;RNA、碱基组分中嘧啶/嘌呤值存在明显差异.这些生化代谢上的差异在组织分化盛期表现最显著.有分化能力的无性系在分化过程中,产生与组织分化有关的蛋白质谱带和过氧化物酶同工酶带,淀粉酶同工酶带与组织分化关系不明显.

Clones with difference level of differentiation have been made in rice callus continuous culture. The analytic results on differentiation medium showed that DNA, RNA and protein content of clones with differentiation capacity are higher significantly than that of these without differentiation. No obvious difference existed between DNase and RNase vigour.However,there are significant difference in (T + C/A+G) value of RNA base composition. The most significant difference of biochemical metabolism occurred in tissue differentiation grand period.Protein, POD zymogram which are relation with tissure differentiation produce in clones with differentiation capacity during continuous culture. No significant relationship existed between amylum zymogram and cell differentiation.