离子色谱柱后衍生法测定汞、锰、镉、钴、锌、镍和铜

Simultaneous Determination of Mercury, Manganese, Cadmium, Cobalt, Zinc, Nickel and Copper by Ion Chromatography With On-column and Post-column Derivatisation

提出了用离子色谱柱后衍生法同时测定汞和其它金属离子的新方法。本方法中样 品离子以阴离子交换分离，用２－（５－溴－２－吡啶偶氮）－５－二乙氨基酚（５－Ｂｒ－ＰＡＤＡＰ）和非离子 表面活性剂 Ｔｒｉｔｏｎ Ｘ－１００显色体系作柱后衍生，分光光度法测定。方法的检出限对 Ｈｇ２＋、 Ｍｎ２＋、Ｃｄ２＋、Ｃｏ２＋、Ｚｎ２＋、Ｎｉ２＋和Ｃｕ２＋分别为２０、９、９、８、２、１００和 ３ μｇ／Ｌ。方法用于人头发样 品中微量汞的分析取得了满意结果。

A new method for simultaneous determination of mercury (Ⅱ) and some other metals by HPIC was established, which involves an on-column derivatisation with 2, 6-pyri- dine-dicarboxylic acid (PDCA ), an anion-exchange separation and a post-column chro- mogenic reaction with 2-[(5-bromo-2-pyridyl)-azo] phenol (5-Br-PADAP). The procedure was optimized with respect to the composition and pH of the eluent and the reagent. The detection limits for Cu2+, Ni2+, Zn2+, Co2+, Cd2+, Mn2+, and Hg2+ are 3, 100, 2, 8, 9, 9, 20 μg/L, respectively. The method has been applied to the determination of mercury in 300 mg human hair sample and the results obtained agree very well with the value announced.