高效液相色谱法测定痕量左旋多巴、甲基多巴、多巴胺和去甲肾上腺素

High Performance Liquid Chromatographic Determination of Trace Amount of Levodopa, Methyldopa, Dopamine and Noradrenaline

以含５％甲醇和０．０２ｍｏｌ／Ｌ邻苯二甲酸氢钾的缓冲液（ｐＨ ３．５２）为流动相，采用安培型电化学检测器，实现了左旋多巴、甲基多巴、多巴胺和去甲肾上腺素在Ｓｈｉｍ－ｐａｃｋ ＣＬＣ－ＯＤＳ（５μｍ，１５０ ｍｍ×６０ｍｍＩ．Ｄ）柱上的完全分离及Ｅ＝＋０．６ Ｖ电压下于玻碳电极上的同时检测。该法线性范围达２个数量级，相关系数大于０．９９９７，最小检出限为 ２８～９４ｐｇ，实验回收率为 ８４．９％～９１．３％。该法快速、灵敏、准确，适用于体液中痕量代谢药物的分析，可用于临床监测。

Trace amount of levodopa, methyldopa, dopamine, and noradrenaline can be determined simultaneously by RP-HPLC on a column of Shim-pack CLC ODS C18, by using a mobile phase of 5% methanol solution and 95% 0.02 mol/L potassium biphthalate buffer(pH 3.52) and an electrochemical detector, at 0.06 V with a detection Limit of 28.0 - 94.0 pg, a linear range of 0.12 - 100 ng, a recovery range of 84.7% - 91.3%, and a correlation coefficient of higher than 0.9997.