氟、钙与碘对大鼠体重的交互作用影响

Interaction of fluorine, calcium and iodine on weight of rats

目的研究氟、钙和碘对大鼠体重的交互作用。方法应用2×2×2析因实验设计将出生1个月的80只Wistar大鼠分成8组，每组10只，分别进行适中氟钙碘（1组）、适中氟碘高钙（2组）、适中钙碘高氟（3组）、适中碘高氟钙（4组）、适中氟钙低碘（5组）、适中氟高钙低碘（6组）、适中钙高氟低碘（7组）、高氟钙低碘（8组）喂养，适中氟为90μg/d，高氟为2700μg／d，适中钙为13mg／d，高钙为260mg／d，适中碘为3．5μg／d，低碘为0．23μg/d。20周后观察其对大鼠体重的影响。结果氟与钙存在交互作用（F=5．933，P=0．017）。喂养满5个月时空腹体重测量结果：适中碘和氟水平时，2组动物体重[（262．5±47．1）g]与1组体重[（307．9±55．0）g]差异有统计学意义（t=4．24，P〈0．05）；低碘和适中氟水平时，6组动物体重[（248．8±30．0）g]与5组体重[（293．3±19．7）g]差异有统计学意义（t=4．16，P〈0．05）；适中碘和钙水平时，低碘和适中氟水平时，3组动物体重[（269．3±27．3）g]与1组体重[（307．9±55．0）g]差异有统计学意义（t=3．61，P〈0．05）；在低碘和适中钙水平时，7组动物体重[（261．9±31．3）g]与5组体重[（293．3±19．7）g]差异有统计学意义（t=2．94，P〈0．05）。结论氟与钙对大鼠体重存在交互作用，可影响大鼠的生长发育。

Objective To study the interaction of fluorine（F）, calcium（Ca） and iodine（I） on body weight of rats. Methods One-month-old Wistar rats were randomly divided into 8 groups ： moderate concentrations of F, Ca, I （ group 1 ） ; moderate concentrations of F, I and high concentration of Ca （ group 2） ; moderate concentrations of Ca, I and high concentration of F（ group 3 ） ; moderate concentration of I and high concentrations of F, Ca（group 4） ; moderate concentrations of F, Ca and low concentration of I（group 5） ; moderate concentration of F,high concentration of Ca and low concentration of I（ group 6） ; moderate concentration of Ca, high concentration of F and low concentration of I （group 7 ）; high concentrations of F, Ca and low concentration of I （ group 8 ） based on 2 × 2 × 2 factorial design. The moderate concentration of F was 90 μg/d and the high concentration of F was 2700 μg/d. The moderate Ca concentration was 13 mg/d and the high Ca concentration was 260 mg/d. The moderate concentration of I was 3.5μg/d and the low concentration of I was 0. 23 μg/d. After twenty weeks, body weight was measured. Results According to the results of factorial ANOVA, significant interaction effects of F with Ca were found（F =5. 933,P =0. 017）. The empty body weight was measured at the end of the fifth month. When both iodine and fluorine were at the optimal level, the weight of group 2 [ （262.5 ± 47.1 ） g ] and group 1[（307.9 ±55.0） g] showed significant difference （t =4.24,P 〈0.05）. When both iodine and fluorine were at low level, the weight of group 6 [ （248.8 ± 30.0） g] and group 5 [ （293.3 ±19.7） g] showed significant difference（t =4.16,P 〈0.05）. Animals with optimal iodine and calcium[ （269.3 ±27.3）g] showed significant difference compared to the weight of low level iodine and optimal fluorine [ （ 307.9 ± 55.0） g]. When the low level iodine and optimal calcium were applied, weight of group7 [ （261.9 ± 31.3 ） gland group 5[（293.3 ± 19.7）g] showed significant difference. （t =2.94,P 〈0.05）. Conclusion Interaction effects of F with Ca were found on body weight in rats.