治疗前后酒精灌胃大鼠创伤弧菌脓毒症肝组织Toll样受体等的变化

Changes of toll-like receptor , TNF-α and IL-10 in liver tissue of rats before and after intragastric infusion with alcohol with vibrio vulnificos sepsis

目的探讨抗生素对酒精灌胃大鼠创伤弧菌（vibrio vulnificus，VV）脓毒症肝组织Toll样受体（Toll like receptor，TLR）等的影响。方法大鼠设正常对照组（N组，6只）、酒精灌胃大鼠对照组（A组，6只）、酒精灌胃大鼠抗生素对照组（AA组，6只）、酒精灌胃大鼠VV脓毒症组（AV组，24只；分别于染菌后2、6、12、24h处死，每时相点动物数均为6只）和酒精灌胃大鼠VV脓毒症抗生素治疗组（AVA组，30只；分别于染菌后6、12、24、36h和1周时活杀，各时相点动物数均为6只）。采用逆转录聚合酶链技术检测各组大鼠不同时点肝组织TLR等的变化。结果AV-6h组TLR4mRNA表达量为0．775±0．101，AVA-6h组TLR4mRNA表达量为0．600±0．064；AV-12h组TLR4mRNA表达量为0．918±0．133，AVA-12h组TLR4mRNA表达量为0．583±0．112；AV-24h组TLR4mRNA表达量为0．732±0．110，AVA-24h组，TLR4mRNA表达量为0．512±0．118。AVA组大鼠染菌后6、12、24hTLR4mRNA，与同时点AV组相比，TLR4mRNA表达量明显降低，差异有统计学意义（AVA-6h组与AV-6h组比较，t=-3．573，P〈0．01；AVA-12h组与AV-12h组比较，t=-4．722，P〈0．01；AVA-24h组与AV-24h组比较，t=-3．340，P〈0．01）。结论及早联用抗生素可减低酒精灌胃大鼠VV脓毒症肝组织TLR等的表达，有利于机体促、抗炎平衡恢复，对酒精灌胃大鼠VV脓毒症有明显的治疗作用。

Objective To detect the effects of antimicrobial agents on the toll-like receptor（TLR） and so on in liver tissue of rats after intragastric infusion with alcohol with vibrio vulnificus （VV） sepsis. Methods Sprague-Dawley rats were randomly divided into normal control group （ N group, n = 6）, rats after intragastric infusion with alcohol control group （ group A, n = 6）, drug intervention on rats after intragastric infusion with alcohol control group （group AA, n = 6）, rats after intragastric infusion with alcohol with VV sepsis group（group AV,n = 24, killed at 2, 6, 12, 24 hours after injecting VV respectively, six rats per group）, as well as drug intervention on rats after intragastric infusion with alcohol with vibrio vulnificus sepsis group （ group AVA, n = 30, killed at 6, 12, 24 hours and one week after injecting VV respectively, six rats per group）. The expressions and dynamic changes of TLR4 mRNA and so on by RT- PCR in liver tissue of each group were measured. Results The expressions of TLR4 mRNA in AV-6 hours group was 0. 775± 0. 101, the expressions of TLR4 mRNA in AVA-6 hours group was 0. 600 ± 0. 064; the expressions of TLR4 mRNA in AV-12 hours group was 0. 918 ±0. 133, the expressions of TLR4 mRNA in AVA-12 hours group was 0. 583 ±0. 112; the expressions of TLR4 mRNA in AV-24 hours group was 0. 732 ±0. 110,the expressions of TLR4 mRNA in AVA-24 hours group was 0. 512 ±0. 118. Compared with AV group, the expressions of TLR4 mRNA in liver diminished greatly in AVA group at 6,12 and 24 hours after being injected with VV（AVA-6 hours group compare with AV-6 hours group, t = -3. 573, P 〈0. 01 ; AVA-12 hours group compared with AV-12 hours group, t = - 4. 722, P 〈 0. 01 ; AVA-24 hours group compare with AV-24 hours group, t = - 3. 340, P 〈 0.01 ）. Conclusion The treatment with antibacterial agents may reduced the expression of TLR and so on in liver of rats after intragastric infusion with alcohol with VV sepsis. The treatment with antibacterial agents may regulate the balance of the inflammatory response in VV sepsis and generate the visible therapeutical effect for VV sepsis.