四种神经肽的反相高效液相色谱法分离

Separation of Four Neuropeptides by Reverse Phase High Performance Liquid Chromatography

以反相高效液相色谱法（ＲＰ－ＨＰＬＣ）分离甲硫脑啡肽（ＭＥＫ）、亮脑啡肽（Ｌｅｕ－ＥＮＫ）、强啡肽Ａ１－１７（ＤｙｎＡ１－１７）、胆囊收缩素（ＣＣＫ－８ｓ）。色谱柱为ＺＯＲＢＡＸＣ１８（２．１ｍｍＩＤ×１５０ｍｍ）；流动相：乙腈／水／三氟乙酸（ＴＦＡ），梯度洗脱，紫外检测器检测：ＵＶ２１５ｎｍ。上述条件下，四种神经肽在微克水平得到较好分离。

Here we report the aeparation of met enkephalin,leu enkephalin,dynorphin A 1-17 and CCK 8s by reverse phase high performance liquid chromatography.Experimental conditions:ZORBAX C 18 reverse phase column,acetonitrile/H 2O/TFA mobile phase ,linear gradient elution,UV detector (UV 215nm).Under these conditions the four neuropeptides can be separated completely.