高效液相色谱法测定新生儿血清中去甲万古霉素浓度

Determination of Demethylvancomycin in Neonate Serum by High Performance Liquid Chromatography

建立了反相高效液相色谱测定新生儿败血症患者血清中去甲万古霉素浓度的方法。血清样品经乙腈－异丙醇沉淀蛋白，上清液用三氯甲烷萃取后以水相进样。流动相为乙腈－磷酸盐缓冲液，紫外检测，最低检测限０．３ｍｇ／Ｌ，有良好的线性关系，回收率达９４．７％，日内、日间精密度分别为２．２３％和２．６２％。结果表明，方法简便、有效。

A rapid high performance liquid chromatography for determination of demethylvancomycin in neonate serum has been developed. The procedure involved a simple protein precipitation by acetonitrileisopropanol(1∶1) and then the sample was chromatographed on a reversed phase C 18 column with UV detection at 236nm. The mobile phase was CH 3CN∶0.05mol/L KH 2PO 4=8∶92(V/V) The calibration curve was Y=35 721.89X-13 031.54, r=0.999 8 and the detection limit was 0.3mg/L The average recovery was 94.7%±12% Intraday and interday RSD were 223% and 2.62% respectively. It can be concluded that this method meets the requirement for routine clinical application. This method has been used to determine serum concentration of demethylvancomycin in neonates. The data obtained showed that the method was simple, rapid, sensitive and precise.