反相高效液相色谱法同时测定番茄中5种类胡萝卜素

Simultaneous Determination of Five Carotenoids in Tomato by Reversed Phase High Performance Liquid Chromatography

用反相高效液相色谱法同时测定了番茄中的α－胡萝卜素、β－胡萝卜素、γ－胡萝卜素、番茄红素及斑蝥黄素。采用１１（Ｖ／Ｖ）丙酮－石油醚混合液直接提取，提取液减压蒸干后，用甲醇溶解，经μ－ＢｏｎｄａｐａｋＣ１８色谱柱，以乙腈－二氯甲烷－甲醇（８５１０５）为流动相，在４５０ｎｍ下进行检测，其保留时间依次为１５．２５，１６．１２，１２．８７，１０．３８和５．３７ｍｉｎ，最小检出限分别为０．２５，０．２２，０．１８，０．１４和０．０５ｍｇ／Ｌ。加标样品的回收率分别为９２．３８％±２．４％，９４．３３％±３．３％，９２．４０％±２．６％，９３．２１％±２．６％和９６．４０％±３．２％。方法简便、快速、准确。

A reversed phase high performance liquid chromatographic method for the simultaneous determination of αcarotene, βcarotene , γcarotene , lycopene and canthaxanthin in tomato has been developed. These carotenoids were extracted with acetone and petroleum ether(1∶1) directly. The extract was dried by N 2 stream. The residue was dissolved in methanol and separated on a μBondapak C18 (Waters) column 150mm×3.9mm i.d., acetonitrile∶ dichloromethane∶methanol(85∶10∶5) as mobile phase. It was then detected at 450nm by UV/VIS spectrophotometer. The retention times of αcarotene, βcarotene , γcarotene, lycopene and canthaxanthin by simultenaneous determination were 15.25, 16.12, 12.87, 10.38 and 5.37min, respectively. Detection limits were 0.25,0.22,0.18,0.14 and 0.05mg/L. Recoveries were 92.38%,94.33%, 92.40%,93.21% and 96.40%, respectively. The coefficients of variation were 2.6%,3.5%,2.8%,2.8% and 3.3%, respectively. The method was simple, rapid and accurate.