高效液相色谱法测定烟叶中茄尼醇的含量

Determination of Solanesol in the Extracts of Tobacco Leaves by High Performance Liquid Chromatography (HPLC)

选用Ｚｏｒｂａｘ－ＳＩＬ（２５０ｍｍ×４．６ｍｍｉ．ｄ．）色谱柱，以正己烷－异丙醚－乙酸乙酯（６３１）混合溶剂作流动相，用示差折光检测器测定了烟叶提取物中茄尼醇的含量，结果表明所用方法具有良好的分离效果和回收率，在０．２～４μｇ之间有良好的线性关系，可用于烟叶或茄尼醇产品中茄尼醇含量的测定。

Solanesol, a terpene compound mainly isolated from tobacco, is an important intermediate of medicines such as vitamin K 2 and coenzyme Q10. This paper presents an HPLC method for the determination of solanesol in the extracts of tobacco leaves. Solanesol is separated completely on a Zorbax SIL column, 250mm×46mm id, by using a mixture of n hexane, isopropyl ether and ethyl acetate (6∶3∶1) as mobile phase and determined refractometrically. The isopropyl ether makes the chromatographic peak sharp and the addition of ethyl acetate can prevent the column from blockade during the analysis, and the appropriate temperature (30℃) is beneficial to the shortening of analysis time. This method is fast, accurate and reproducible. It has good linear relationship in the range of 0.2 4μg. The results have shown that the average recovery is 96.4% and coefficient of variation is 2.8%.