离子注入对黑松花粉粒和花粉管内骨架系统的损伤效应

Effects on Cytoskeleton System in Pollen Tube of Pinus thunbergii Induced by Ion Beam Implantation

以黑松花粉粒和花粉管为试验材料,研究了离子注入对细胞骨架系统的损伤效应。研究结果表明,离子注入会不同程度地破坏花粉管内微管网络的完整性,花粉管形态的异常状态与其微管骨架结构的异常状态密切相关。离子注入对黑松花粉管内的微管骨架系统的正常结构有明显的效应,这种效应的明显程度与离子注入剂量有一定的相关性,即随着离子注入剂量的增加,微管骨架系统受到破坏的程度更加明显。离子注入对黑松花粉管内微丝骨架系统的分布状态有明显的影响,其程度也与离子注入剂量的大小存在一定的相关性。

The damage of the cytoskeleton system in the pollen and the pollen tube of Pinus thunbergii in -duced by ion beam implantation were researched. The results showed that the disorganization of the microtubules in the pollen tube was produced by N^＋ implantation. The abnormal states of the pollen tube in morphology were very correlative with the abnormality of the cytoskeleton system. N^＋ implantation was responsible for morphological abnormalities in the pollen tubes. There was a distinct correlation between the damage effects and the ion implantation dose. The add of dose caused more obvious damage effects. Furthermore, the state of the cytoskeleton system in the pollen tube was influenced by the ion implantation. The impact grade depended also on the ion implantation dose.