大鼠异位心脏移植模型手术的改良

Modified surgical technique for heterotopic rat heart transplantation model

目的对大鼠腹部异位心脏移植模型进行操作改良,总结手术技巧以提高手术成功率。方法以Wistar大鼠作供体,SD大鼠作为受体,常规组供心升主动脉与受体腹主动脉吻合,肺动脉与下腔静脉吻合;改良组采用供心升主动脉与受体腹主动脉吻合,肺动脉与左肾静脉端侧吻合完成心脏移植。术后受体鼠经胃管内给予10mg/(kg·d)环孢菌素A,比较两组的手术时间、手术成功率和长期存活率。结果改良组和常规组手术时间分别为(36.8±5.6)min和(40.7±6.5)min,两者相比差异有显著性(P<0.05)。改良组的手术成功率为90%,明显高于常规组的65%(P<0.05);移植成功后常规组和改良组术后长期存活率和供心存活时间之间无显著性差异(P<0.05)。结论改良组手术时间短、成功率高,值得进一步推广。

Objective The aim of this study was to modify the abdominal heterotopic heart transplantation model for experimental studies. Methods Wistar rats were used as donors and Spr.ague-Dawley rats as recipients. In normal group, the ascending aorta of the donor was sutured end-to-side to the abdominal aorta of the recipient, and the pulmonary artery of the donor was anastomosed to the inferior vena cava. In modified group, the ascending aorta of the donor was sutured end-to-side to the abdominal aorta of the recipient, and the pulmonary artery of the donor was anastomosed to the left renal vein of the recipient. CsA [10 mg/（kg.day）] was administered orally to the recipient rats after operation. The operation time, the successful rates and the long-time survival rates were compared between two groups. Results Compared with the normal group, the modified group had less operation time [（36.8±5.6） vs （40.7±6.5） min, P 〈0.05] and higher successful operation rates （90% vs 65%, P 〈0.05）. The long-term survival rates were similar in modified and normal groups： 80.6% and 76.9% respectively, and the survival time had no statistically significant difference between two groups. Conclusion The modified procedure saves operation time and has lower incidence of post-operation mortality. The technique is valuable for heterotopic heart transplantation in rats.