膀胱癌中Smad4、Ki-67、CyclinD1基因表达及临床意义

Clinical significance of Smad4,Ki-67 and CyclinD1 genes expression in bladder carcinoma

目的检测Smad4、Ki-67、CyclinD1基因在膀胱癌中的表达并探讨其临床意义。方法采用RT- PCR技术检测32例膀胱移行细胞癌癌组织、癌旁组织及正常膀胱组织中Smad4、Ki-67、CyclinD1基因的表达,结合临床资料分析评价上述基因表达与膀胱癌临床生物学行为的关系。结果Smad4、Ki-67、CyclinD1在膀胱癌组织阳性表达率分别为34.4%、53.1%、46.9%(P<0.05)。Smad4的表达随膀胱癌病理分级、临床分期的升高而下降(P<0.05),而Ki-67的表达呈递增趋势(P<0.01)。CyclinD1在正常组织中不表达,而在G1期呈高表达,并且随膀胱癌病理分级、临床分期的增加呈递减趋势(P<0.01)。Smad4与Ki-67的表达呈负相关(r=-0.673,P<0.05),Smad4与CyclinD1呈正相关(r=0.717,P<0.05),Ki-67与CyclinD1呈负相关(r=-0.515,P<0.05)。结论Ki-67、CyclinD1基因的表达异常及协同作用在膀胱癌的发生和发展过程中起重要作用。

Objective To investigate the gene expressions of Smad4, Ki-67 and CyclinD1 in human bladder transitional cell carcinoma and their significance. Methods Smad4, Ki-67 and CyclinD1 genes were examined in 32 cases of bladder carcinoma, adjacent and normal tissue by reverse transcriptase polymerase chain reaction assay, then the correlation between these gene expressions and biological behaviors of bladder carcinoma was evaluated, Results The expres- sions of Smad4, Ki-67 and CyclinD1 mRNA in bladder carcinoma tissue were 34.4%, 53.1% and 46.9%（P 〈0.05）, respectively. Smad4 expression was declined with the increasing tumor grade and stage （P 〈0.05）, but Ki-67 expression was increased （P 〈0.01）. CyclinD1 did not express in the normal groups, highly expressed in C1, and decreased with the increasing tumor grade and stage （P 〈0.01）. A significant negative correlation was observed between the expressions of Smad4 and Ki-67（r=-0.673, P 〈0.05）, CyclinD1 was positively correlated with the expression of Smad4（r=0.717, P 〈0.05）, Ki-67 was negative correlation with the expression of CyclinDl（r = -0.515, P 〈0.05）. Conclusion It is suggested that abnormal expression and synergism of Smad4, Ki-67 and CyclinD1 mRNA may play an important role in the genesis and malignant progression of bladder carcinoma.