青蛤北方3个群体遗传多样性分析

RAPD Analysis of Three Geographical Stocks of Clam Cyclina sineusis

采用随机扩增多态性DNA（RAPD）技术对辽宁省大连庄河海区、锦州海区及山东省东营海区青蛤群体的遗传结构和遗传变异进行分析。15条随机引物共检测到123个位点（250～2000bp），3个群体的青蛤最大遗传距离为0.0549，遗传相似性系数超过94％，群体分化不明显，未形成不同的地理种群。3个群体总的DNA多态位点百分率为94．2％，表明3个群体青蛤当前种质资源状况良好，遗传多样性水平较高。聚类分析显示辽宁锦州群体和山东东营群体优先聚类，亲缘关系较近。

RAPD was conducted to analyse the genetic variation and genetic structure of clam Cyclina sineusis in 3 geographical stocks from Zhuanghe and Jinzhou（LJ） in Liaoning province and Dongying（SD） in Shandong province. The result showed that a total of 123 loci were detected ranging from 250 bp to 2 000 bp with 15 random primers. Among the 3 geographical stocks, the maximum genetic distance was 0.0549, and the genetic resemblance attained more than 94% , so the population differentiation was not obvious, and geographical stocks did not exist. There was a total of 94.2% DNA polymorphic site in the 3 populations, showing the well germplasm resource, and the high genetic diversity level. Clustering analysis showed that the closest phylogenetic relationship occured between the SD and LJ which had closer genetic relationship.