液相色谱-质谱联用法快速筛选苔类粗提液中双联苄化合物

Rapid Screening for Bisbibenzyls in Bryophyte Crude Extracts Using Liquid Chromatography-Tandem Mass Spectrometry

采用电喷雾三重四极杆质谱(ESI-TQ-MS)以负离子扫描方式对15种双联苄对照品进行质谱研究,通过比较质谱碎片信息,对其质谱规律进行解析,并采用液相色谱-电喷雾串联质谱(LC-ESI-MS/MS)法分析地钱(Marchantia polymorphaL.)和花萼苔(Asterella angusta)两种苔类粗提液,快速筛选其中的双联苄类化合物。通过比较色谱和质谱行为特征,并结合紫外吸收光谱数据,分别检测出5/18种和8/16种已知/未知双联苄化合物。该法选择性强、灵敏度高,丰富了对双联苄类化合物裂解规律的研究,并为筛选其他结构类似物提供了参考。

Liquid chromatography coupled with electrospray ionization tandem mass spectrometry （LC-ESI/MS/MS） in negative ion mode was used for the screening of bisbibenzyls in 2 bryophyte crude extracts （Marchantia polymorpha L. and Asterella angusta）. To obtain the mass spectrometric characteristics of bisbibenzyls, 15 confirmed bisbibenzyl compounds were investigated, and the fragmentation patterns were proposed. Further characterization of each crude extract was performed using full scan and MS/MS product ion scan analysis. The confirmed or unconfirmed bisbibenzyls were identified or proposed by comparison of the retention behavior, mass spectrometric data and the UV spectroscopic data with confirmed bisbibenzyl compounds. As a result, 5/18, 8/16 confirmed/unconfirmed bisbiber/zyls are determined in the two crude extracts respectively. The method is simple, reliable and sensitive, and it can be used for screening other bisbibenzyl analogs.