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姜黄素对PC12细胞蛋白酶体活性的双相作用

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摘要 目的研究不同浓度姜黄素(curcumin,Cur)对PC12细胞蛋白酶体活性的影响。方法用四氮唑盐(MTT)法检测细胞活力、荧光分光光度计检测蛋白酶体糜蛋白酶样水解酶活性。结果0.5μmol和1.0μmol的姜黄素对PC12细胞活力无影响,5μmol和10μmol的姜黄素使细胞活力分别下降28.6%和48.5%;0.5μmol和1.0μmol的姜黄素使糜蛋白酶样水解酶活性分别增加31%和44%,而5μmol和10μmol的姜黄素使糜蛋白酶样活性分别下降32%和45%。结论低浓度姜黄素诱导蛋白酶体活性,可能通过此途径保护多巴胺能神经元。
作者 崔群力
出处 《中国实用神经疾病杂志》 2008年第10期108-109,共2页 Chinese Journal of Practical Nervous Diseases
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