谷氨酸钠对胎鼠脑发育影响的实验研究

Experimental Study on the Influence of Sodium Glutamate on Embryonal Brain Development of Mouse

味精的主要成份谷氨酸钠，经代谢产生的谷氨酸是一典型的兴奋性氨基酸，其神经毒性作用已受到广泛关注。本实验采用健康昆明种小白鼠，从妊娠后的第４天开始腹膜腔注射谷氨酸钠（剂量分别为６０、３０、１０ｍｇ／ｋｇ，隔日一次）。于妊娠后的第１８天检查胎鼠，取脑Ｃａｒｎｏｒｙ＇ｓ液固定，常规石蜡切片，ＨＥ、Ｆｅｕｌｇｅｎ和Ｎｉｓｌ染色。进行形态观察，图像分析仪随机测定大脑皮质１００个神经元的Ｆｅｕｌｇｅｎ与Ｎｉｓｌ染色的灰度值，作定量分析。结果显示：各实验组与对照组均未见形态异常。实验组各项参数与对照组比较Ｐ＞０．０５。本实验结果提示：谷氨酸钠对胎鼠脑发育无不良影响。

The main component of gourment powder is a typical excitatory amino acid.Its neurotoxic action has been attended extensively. Healthy Kunming strain mice were used in this study.Sodium glutamate was injected into peritoneal cavity from the fourth day of gestation (at dosages of 60,30 or 10mg/kg every other day).The embryonal mice were dissected at the eighteenth day of gestation.The brain of embryonal mouse was removed,weighed, and fixed in Carnory's solution.Routine paraffin section was made .HE,Feulgen and Nissl staining methods were used for morphological observation.The ash values of 100 neurons of cerebral cortex of Feulgen and Nissl staining were randomly measured with an automated image analyser.The results showed that there was no morphological change in the experimental and control group.There was no significant differece between experimental groups and control group in the parameters observed ( P >0 05).It was suggested that sodium glutamate was not harmful to embryonal brain development of mouse.