牛磺酸对小鼠移植瘤S_(180)肉瘤影响的研究

Research on Effects of Taurine on the Transplanted Tumor of Mice

小鼠喂以基础饲料，对照组和实验组两组动物分别饮水和１％牛磺酸，进行下列实验：１．接种Ｓ１８０肉瘤后，各组又分为不注射环磷酰胺（ＣＴＸ）和注射环磷酰胺进行化疗两组。观察小鼠存活时间、肉瘤大小及抑瘤率。结果表明：补充牛磺酸的荷瘤小鼠存活时间（３５．７±２３．７）天，明显长于对照组（１８．７±５．６）天，抑瘤率为４２．２６％，牛磺酸与ＣＴＸ合用的小鼠生存至６１天仍无一例死亡，也未见到肉眼肿块，抑瘤率为１００％。对照组用ＣＴＸ小鼠存活时间（３９．８±１２．３）天，与未用ＣＴＸ小鼠无差别。２．小鼠接种艾氏腹水瘤瘤细胞后，观察瘤细胞的膜流动性。结果表明：接种艾氏腹水瘤后，牛磺酸组瘤细胞膜的荧光偏振度ｐ＝０．１０８±０．０２０、微粘度η＝０．６１８±０．１４０；而对照组ｐ＝０．１３９±０．０２０，η＝０．８７４±０．１５０，说明牛磺酸组细胞膜的流动性明显优于对照组（Ｐ＜０．０１）。３．以碳素廓清法和血清溶血素生成测定两组免疫功能。牛磺酸组小鼠的免疫功能也明显高于对照组（Ｐ＜０．０１）。以上结果证明：牛磺酸具有抑瘤效果，在用ＣＴＸ进行化疗时，具有明显的协同效应。其抑瘤效果可能与牛磺酸能加强机体免疫功能、保护细胞膜的稳定性和?

With the same forage to the mice,three experiments were done.The control group took water and experimental group took 1% taurine.1.After the mice were vaccinated with S 180 sarcoma,every group was further divided into two groups,one treated with cytoxan (CTX), another one treated with nothing.The survival period,weight of the tumor and the tumor inhibition rate were investigated.The result shows:The survival period of the group fed with taurine was (35 75± 23 7 ) days longer than that of the control group (18.7±5.6) days,and the tumor inhibition rate was 42.26%.The mice fed with taurine and treated with CTX survived to 61 days with no death,and no tumor could be seen grossly,so the tumor inhibition rate is 100%.The survival period and weight of the tumor were not different between the CTX treated and control groups.2.Tumor cell membrane fluidity was observed after the mice were vaccinated with Erlish ascitic tumor.The result shows:in the taurine group,the fluorescence polarization ( p ) is 0.108±0.020 ,the viscosity ( η ) is 0.618±0.140 and in the control group p is 0.139 ±0.020, η is 0.874±0.150, respectively.Thus the cell membrane fluidity of the taurine group is also obviously better than that of the control group ( P <0 01).3.The immune functions of the two groups were determined by carbon clearance test and capacity of serum hemolysin.The immune function of the taurine group is also obviously higher than that of control group ( P <0 01).All the results described above show:taurine is functioning for tumor inhibition,and has obvious synergic effect while treated with CTX as chemotherophy.It is considered that the tumor inhibit function of taurine may be related to its effects of immune enhancing and membrane profecting.